SIT4/YDL047W Summary Help

Standard Name SIT4 1
Systematic Name YDL047W
Alias PPH1 2
Feature Type ORF, Verified
Description Type 2A-related serine-threonine phosphatase; functions in the G1/S transition of the mitotic cycle; regulator of COPII coat dephosphorylation; required for ER to Golgi traffic; interacts with Hrr25p kinase; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization; similar to human PP6 (3, 4, 5, 6, 7, 8)
Name Description Suppressor of Initiation of Transcription 1
Chromosomal Location
ChrIV:369771 to 370706 | ORF Map | GBrowse
Gene Ontology Annotations All SIT4 GO evidence and references
  View Computational GO annotations for SIT4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
Large-scale survey
279 total interaction(s) for 167 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 97
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 25
  • Biochemical Activity: 2
  • Co-fractionation: 2
  • Co-purification: 2
  • PCA: 2
  • Reconstituted Complex: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 38
  • Negative Genetic: 13
  • Phenotypic Enhancement: 10
  • Phenotypic Suppression: 14
  • Positive Genetic: 19
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 26
  • Synthetic Rescue: 17

Expression Summary
Length (a.a.) 311
Molecular Weight (Da) 35,537
Isoelectric Point (pI) 5.17
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:369771 to 370706 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..936 369771..370706 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002205
References cited on this page View Complete Literature Guide for SIT4
1) Arndt KT, et al.  (1989) A suppressor of a HIS4 transcriptional defect encodes a protein with homology to the catalytic subunit of protein phosphatases. Cell 56(4):527-37
2) Ronne H, et al.  (1991) Protein phosphatase 2A in Saccharomyces cerevisiae: effects on cell growth and bud morphogenesis. Mol Cell Biol 11(10):4876-84
3) Stark MJ  (1996) Yeast protein serine/threonine phosphatases: multiple roles and diverse regulation. Yeast 12(16):1647-75
4) Sutton A, et al.  (1991) The SIT4 protein phosphatase functions in late G1 for progression into S phase. Mol Cell Biol 11(4):2133-48
5) Zabrocki P, et al.  (2002) Protein phosphatase 2A on track for nutrient-induced signalling in yeast. Mol Microbiol 43(4):835-42
6) Angeles de la Torre-Ruiz M, et al.  (2002) Sit4 is required for proper modulation of the biological functions mediated by Pkc1 and the cell integrity pathway in Saccharomyces cerevisiae. J Biol Chem 277(36):33468-76
7) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
8) Bhandari D, et al.  (2013) Sit4p/PP6 regulates ER-to-Golgi traffic by controlling the dephosphorylation of COPII coat subunits. Mol Biol Cell 24(17):2727-38