SIN4/YNL236W Summary Help

Standard Name SIN4 1, 2
Systematic Name YNL236W
Alias BEL2 , GAL22 , SDI3 3 , SSF5 , SSN4 , TSF3 3 , RYE1 , MED16 4 , SSX3 5
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription (6, 7)
Name Description Switch INdependent
Chromosomal Location
ChrXIV:206930 to 209854 | ORF Map | GBrowse
Gbrowse
Genetic position: -145 cM
Gene Ontology Annotations All SIN4 GO evidence and references
  View Computational GO annotations for SIN4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 387 genes
Regulators 1 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
overexpression
Resources
530 total interaction(s) for 386 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 80
  • Affinity Capture-Western: 21
  • Co-purification: 9
  • Two-hybrid: 13

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 5
  • Negative Genetic: 252
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 11
  • Positive Genetic: 69
  • Synthetic Growth Defect: 24
  • Synthetic Lethality: 16
  • Synthetic Rescue: 23

Resources
Expression Summary
histogram
Resources
Length (a.a.) 974
Molecular Weight (Da) 111,296
Isoelectric Point (pI) 8.78
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:206930 to 209854 | ORF Map | GBrowse
SGD ORF map
Genetic position: -145 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2925 206930..209854 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005180
References cited on this page View Complete Literature Guide for SIN4
1) Jiang YW and Stillman DJ  (1992) Involvement of the SIN4 global transcriptional regulator in the chromatin structure of Saccharomyces cerevisiae. Mol Cell Biol 12(10):4503-14
2) Chen, S.  (1992) Personal Communication, Mortimer Map Edition 11
3) Stillman DJ, et al.  (1994) Epistasis analysis of suppressor mutations that allow HO expression in the absence of the yeast SW15 transcriptional activator. Genetics 136(3):781-8
4) Bourbon HM, et al.  (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
5) Li L, et al.  (2005) Genetic interactions between mediator and the late G1-specific transcription factor Swi6 in Saccharomyces cerevisiae. Genetics 171(2):477-88
6) Li Y, et al.  (1995) Yeast global transcriptional regulators Sin4 and Rgr1 are components of mediator complex/RNA polymerase II holoenzyme. Proc Natl Acad Sci U S A 92(24):10864-8
7) Myers LC and Kornberg RD  (2000) Mediator of transcriptional regulation. Annu Rev Biochem 69():729-49