SIL1/YOL031C Summary Help

Standard Name SIL1 1
Systematic Name YOL031C
Alias SLS1 2
Feature Type ORF, Verified
Description Nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; required for protein translocation into the endoplasmic reticulum (ER); homolog of Yarrowia lipolytica SLS1; GrpE-like protein (1, 2, 3)
Name Description Suppressor of the Ire1/Lhs1 double mutant 1
Chromosomal Location
ChrXV:267529 to 266264 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SIL1 GO evidence and references
  View Computational GO annotations for SIL1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
unspecified
Resources
118 total interaction(s) for 61 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-Western: 5
  • Co-crystal Structure: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 33
  • Phenotypic Enhancement: 32
  • Phenotypic Suppression: 14
  • Positive Genetic: 13
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 5
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 421
Molecular Weight (Da) 48,274
Isoelectric Point (pI) 5.32
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:267529 to 266264 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1266 267529..266264 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005391
References cited on this page View Complete Literature Guide for SIL1
1) Tyson JR and Stirling CJ  (2000) LHS1 and SIL1 provide a lumenal function that is essential for protein translocation into the endoplasmic reticulum. EMBO J 19(23):6440-52
2) Kabani M, et al.  (2000) Sls1p stimulates Sec63p-mediated activation of Kar2p in a conformation-dependent manner in the yeast endoplasmic reticulum. Mol Cell Biol 20(18):6923-34
3) Kabani M, et al.  (2002) HspBP1, a homologue of the yeast Fes1 and Sls1 proteins, is an Hsc70 nucleotide exchange factor. FEBS Lett 531(2):339-42