SIF2/YBR103W Summary Help

Standard Name SIF2 1
Systematic Name YBR103W
Alias EMB1 2
Feature Type ORF, Verified
Description WD40 repeat-containing subunit of Set3C histone deacetylase complex; complex represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus (1, 3, 4)
Name Description Sir4p-Interacting Factor 3
Chromosomal Location
ChrII:447709 to 449316 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SIF2 GO evidence and references
  View Computational GO annotations for SIF2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Classical genetics
null
overexpression
unspecified
Large-scale survey
null
Resources
357 total interaction(s) for 215 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 56
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 3
  • Two-hybrid: 11

Genetic Interactions
  • Negative Genetic: 174
  • Phenotypic Enhancement: 11
  • Phenotypic Suppression: 17
  • Positive Genetic: 37
  • Synthetic Growth Defect: 33
  • Synthetic Lethality: 8
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 535
Molecular Weight (Da) 59,161
Isoelectric Point (pI) 4.66
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:447709 to 449316 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1608 447709..449316 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000307
References cited on this page View Complete Literature Guide for SIF2
1) Gasser SM, et al.  (1998) Nuclear organization and silencing: trafficking of Sir proteins. Novartis Found Symp 214():114-26; discussion 126-32
2) Entian KD, et al.  (1999) Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach. Mol Gen Genet 262(4-5):683-702
3) Cockell M, et al.  (1998) Sif2p interacts with Sir4p amino-terminal domain and antagonizes telomeric silencing in yeast. Curr Biol 8(13):787-90
4) Pijnappel WW, et al.  (2001) The S. cerevisiae SET3 complex includes two histone deacetylases, Hos2 and Hst1, and is a meiotic-specific repressor of the sporulation gene program. Genes Dev 15(22):2991-3004