SHR5/YOL110W Summary Help

Standard Name SHR5 1
Systematic Name YOL110W
Alias ERF4 2
Feature Type ORF, Verified
Description Palmitoyltransferase subunit; this complex adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization; Palmitoyltransferase is composed of Shr5p and Erf2 (3, 4, 5)
Name Description Suppressor of Hyperactive Ras 1
Chromosomal Location
ChrXV:109176 to 109889 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SHR5 GO evidence and references
  View Computational GO annotations for SHR5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
48 total interaction(s) for 33 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 1
  • PCA: 1
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 19
  • Phenotypic Suppression: 4
  • Positive Genetic: 10
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 3
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 237
Molecular Weight (Da) 26,541
Isoelectric Point (pI) 4.99
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:109176 to 109889 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..714 109176..109889 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005470
References cited on this page View Complete Literature Guide for SHR5
1) Jung V, et al.  (1995) Mutations in the SHR5 gene of Saccharomyces cerevisiae suppress Ras function and block membrane attachment and palmitoylation of Ras proteins. Mol Cell Biol 15(3):1333-42
2) Bartels DJ, et al.  (1999) Erf2, a novel gene product that affects the localization and palmitoylation of Ras2 in Saccharomyces cerevisiae. Mol Cell Biol 19(10):6775-87
3) Zhao L, et al.  (2002) Erf4p and Erf2p form an endoplasmic reticulum-associated complex involved in the plasma membrane localization of yeast Ras proteins. J Biol Chem 277(51):49352-9
4) Dong X, et al.  (2003) Palmitoylation and plasma membrane localization of Ras2p by a nonclassical trafficking pathway in Saccharomyces cerevisiae. Mol Cell Biol 23(18):6574-84
5) Roth AF, et al.  (2006) Global analysis of protein palmitoylation in yeast. Cell 125(5):1003-13