SHO1/YER118C Summary

SHO1/YER118C Summary

Standard Name	SHO1
Systematic Name	YER118C
Alias	SSU81
Feature Type	ORF, Verified
Description	Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p (1, 2, 3, 4, 5, 6, 7)

Chromosomal Location	ChrV:399055 to 397952 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All SHO1 GO evidence and references

	View Computational GO annotations for SHO1
Molecular Function
Manually curated	MAP-kinase scaffold activity (IMP, IPI) osmosensor activity (IPI)
Biological Process
Manually curated	cellular response to heat (IMP) cytokinesis (IGI, IMP, IPI) establishment of cell polarity (IGI) osmosensory signaling pathway (IGI, IPI) signal transduction involved in filamentous growth (IMP)
Cellular Component
Manually curated	cellular bud (IDA) cellular bud neck (IDA) HICS complex (IPI) mating projection tip (IDA) plasma membrane part (IDA)
High-throughput	integral to membrane (ISM)

Regulatory Role

Regulatory modules	predicted: stressResponse (487, 481, 463) predicted: cellcycle (359, 215) predicted: stressResponse (487, 481, 463) predicted: cellcycle (359, 215)

Mutant phenotypes All SHO1 Phenotype evidence and references

Classical genetics
null	invasive growth: decreased pseudohyphal growth: decreased resistance to Calcofluor White: increased resistance to enzymatic treatment: decreased
Large-scale survey
null	auxotrophy competitive fitness: increased haploinsufficient resistance to paromomycin: decreased resistance to glycolaldehyde: decreased resistance to cycloheximide: decreased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased vacuolar morphology: abnormal viable
overexpression	cell cycle progression: abnormal resistance to sirolimus: decreased vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All SHO1 Interaction evidence and references

	207 total interaction(s) for 133 unique genes/features.
Physical Interactions	Affinity Capture-RNA: 1 Affinity Capture-Western: 15 Biochemical Activity: 1 PCA: 29 Protein-peptide: 1 Reconstituted Complex: 11 Two-hybrid: 52
Genetic Interactions	Dosage Growth Defect: 1 Negative Genetic: 63 Phenotypic Enhancement: 6 Phenotypic Suppression: 9 Positive Genetic: 1 Synthetic Growth Defect: 7 Synthetic Haploinsufficiency: 2 Synthetic Lethality: 6 Synthetic Rescue: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All SHO1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrV:399055 to 397952 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1104	399055..397952	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000920

References cited on this page View Complete Literature Guide for SHO1

1)	Posas F and Saito H (1997) Osmotic activation of the HOG MAPK pathway via Ste11p MAPKKK: scaffold role of Pbs2p MAPKK. Science 276(5319):1702-5
2)	Zarrinpar A, et al. (2003) Optimization of specificity in a cellular protein interaction network by negative selection. Nature 426(6967):676-80
3)	O'Rourke SM and Herskowitz I (2004) Unique and redundant roles for HOG MAPK pathway components as revealed by whole-genome expression analysis. Mol Biol Cell 15(2):532-42
4)	Cullen PJ, et al. (2004) A signaling mucin at the head of the Cdc42- and MAPK-dependent filamentous growth pathway in yeast. Genes Dev 18(14):1695-708
5)	Tatebayashi K, et al. (2006) Adaptor functions of Cdc42, Ste50, and Sho1 in the yeast osmoregulatory HOG MAPK pathway. EMBO J 25(13):3033-44
6)	Hao N, et al. (2007) A systems-biology analysis of feedback inhibition in the Sho1 osmotic-stress-response pathway. Curr Biol 17(8):659-67
7)	Tatebayashi K, et al. (2007) Transmembrane mucins Hkr1 and Msb2 are putative osmosensors in the SHO1 branch of yeast HOG pathway. EMBO J 26(15):3521-33