SHE4/YOR035C Summary

Standard Name	SHE4 1 (see Nomenclature conflict Note)
Systematic Name	YOR035C
Alias	DIM1 2
Feature Type	ORF, Verified
Description	Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization (3, 4)
Name Description	Swi5p-dependent HO Expression 1

Chromosomal Location	ChrXV:400104 to 397735 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All SHE4 GO evidence and references

	View Computational GO annotations for SHE4
Molecular Function
Manually curated	myosin binding (IDA)
Biological Process
Manually curated	actin cytoskeleton organization (TAS) intracellular mRNA localization (IMP) mating type switching (IMP)
Cellular Component
Manually curated	cytoplasm (IDA)

Mutant phenotypes All SHE4 Phenotype evidence and references

Classical genetics
null	budding pattern: abnormal cell shape: abnormal tellurium atom accumulation: decreased filamentous growth: abnormal resistance to 6-hydroxylaminopurine: decreased
Large-scale survey
null	UV resistance: decreased acid pH resistance: decreased bud morphology: abnormal cell size: increased chitin deposition: increased competitive fitness: decreased dessication resistance: decreased endocytosis: decreased fermentative growth: decreased rate haploproficient heat sensitivity: increased mutation frequency: decreased Fus-Mid-GFP distribution: abnormal resistance to caffeine: decreased resistance to ethanol: decreased resistance to propan-1-ol: decreased resistance to acetaldehyde: decreased resistance to hydroxyurea: decreased resistance to monensin: decreased resistance to Calcofluor White: decreased resistance to methyl methanesulfonate: increased resistance to cycloheximide: increased resistance to hydrogen chloride: decreased resistance to quinine: decreased resistance to bleomycin: decreased resistance to camptothecin: decreased resistance to fenpropimorph: decreased resistance to mycophenolic acid: decreased resistance to dimethyl sulfoxide: decreased resistance to fluconazole: decreased resistance to sirolimus: decreased resistance to acetic acid: decreased resistance to (S)-lactic acid: decreased resistance to wortmannin: decreased resistance to benomyl: decreased resistance to cycloheximide: decreased resistance to hygromycin B: decreased resistance to benzo[a]pyrene: decreased resistance to glycolaldehyde: decreased sporulation efficiency: increased stress resistance: increased toxin resistance: decreased transposable element transposition: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All SHE4 Interaction evidence and references

	323 total interaction(s) for 216 unique genes/features.
Physical Interactions	Affinity Capture-MS: 7 Affinity Capture-RNA: 1 Affinity Capture-Western: 4 Biochemical Activity: 1 Co-crystal Structure: 1 Co-fractionation: 2 Reconstituted Complex: 3 Two-hybrid: 8
Genetic Interactions	Dosage Lethality: 2 Negative Genetic: 236 Phenotypic Enhancement: 2 Positive Genetic: 9 Synthetic Growth Defect: 30 Synthetic Lethality: 16 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All SHE4 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXV:400104 to 397735 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2006-01-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2370	400104..397735	2006-01-05	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005561

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
DIM1	Nomenclature conflict	Both DIM1/YPL266W and SHE4/YOR035C have been referred to as DIM1

References cited on this page View Complete Literature Guide for SHE4

1)	Jansen RP, et al. (1996) Mother cell-specific HO expression in budding yeast depends on the unconventional myosin myo4p and other cytoplasmic proteins. Cell 84(5):687-97
2)	Wendland B, et al. (1996) A novel fluorescence-activated cell sorter-based screen for yeast endocytosis mutants identifies a yeast homologue of mammalian eps15. J Cell Biol 135(6 Pt 1):1485-500
3)	Toi H, et al. (2003) She4p/Dim1p interacts with the motor domain of unconventional myosins in the budding yeast, Saccharomyces cerevisiae. Mol Biol Cell 14(6):2237-49
4)	Wesche S, et al. (2003) The UCS domain protein She4p binds to myosin motor domains and is essential for class I and class V myosin function. Curr Biol 13(9):715-24