SHE2/YKL130C Summary Help

Standard Name SHE2 1
Systematic Name YKL130C
Feature Type ORF, Verified
Description RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; binds to ER-derived membranes and targets mRNAs to cortical ER (2, 3, 4, 5, 6, 7)
Name Description Swi5p-dependent HO Expression 1
Chromosomal Location
ChrXI:196028 to 195288 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SHE2 GO evidence and references
  View Computational GO annotations for SHE2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
142 total interaction(s) for 88 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 21
  • Affinity Capture-RNA: 59
  • Affinity Capture-Western: 14
  • Co-crystal Structure: 4
  • Co-fractionation: 2
  • PCA: 2
  • Protein-peptide: 1
  • Protein-RNA: 8
  • Reconstituted Complex: 9
  • Two-hybrid: 13

Genetic Interactions
  • Negative Genetic: 6
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 246
Molecular Weight (Da) 28,251
Isoelectric Point (pI) 4.7
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:196028 to 195288 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..741 196028..195288 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001613
References cited on this page View Complete Literature Guide for SHE2
1) Jansen RP, et al.  (1996) Mother cell-specific HO expression in budding yeast depends on the unconventional myosin myo4p and other cytoplasmic proteins. Cell 84(5):687-97
2) Takizawa PA and Vale RD  (2000) The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p. Proc Natl Acad Sci U S A 97(10):5273-8
3) Long RM, et al.  (2000) She2p is a novel RNA-binding protein that recruits the Myo4p-She3p complex to ASH1 mRNA. EMBO J 19(23):6592-601
4) Bohl F, et al.  (2000) She2p, a novel RNA-binding protein tethers ASH1 mRNA to the Myo4p myosin motor via She3p. EMBO J 19(20):5514-24
5) Shepard KA, et al.  (2003) Widespread cytoplasmic mRNA transport in yeast: identification of 22 bud-localized transcripts using DNA microarray analysis. Proc Natl Acad Sci U S A 100(20):11429-34
6) Estrada P, et al.  (2003) Myo4p and She3p are required for cortical ER inheritance in Saccharomyces cerevisiae. J Cell Biol 163(6):1255-66
7) Genz C, et al.  (2013) Association of the Yeast RNA-binding Protein She2p with the Tubular Endoplasmic Reticulum Depends on Membrane Curvature. J Biol Chem 288(45):32384-93