SGF73/YGL066W Summary Help

Standard Name SGF73 1
Systematic Name YGL066W
Alias SCA7 2
Feature Type ORF, Verified
Description SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; relocalizes to the cytosol in response to hypoxia; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay (1, 2, 3, 4, 5, 6, 7, 8)
Name Description SaGa associated Factor, 73 kDa 1
Chromosomal Location
ChrVII:377609 to 379582 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SGF73 GO evidence and references
  View Computational GO annotations for SGF73
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All SGF73 Phenotype evidence and references
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
interactions All SGF73 Interaction evidence and references
591 total interaction(s) for 350 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 186
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 17
  • Biochemical Activity: 6
  • Reconstituted Complex: 1
  • Two-hybrid: 5
Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 272
  • Phenotypic Suppression: 1
  • Positive Genetic: 43
  • Synthetic Growth Defect: 48
  • Synthetic Lethality: 7
  • Synthetic Rescue: 1
Resources
Expression Summary
histogram
Resources
Protein Information All SGF73 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:377609 to 379582 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1974 377609..379582 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003034
References cited on this page View Complete Literature Guide for SGF73
1) Sanders SL, et al.  (2002) Proteomics of the eukaryotic transcription machinery: identification of proteins associated with components of yeast TFIID by multidimensional mass spectrometry. Mol Cell Biol 22(13):4723-38
2) McMahon SJ, et al.  (2005) Polyglutamine-expanded spinocerebellar ataxia-7 protein disrupts normal SAGA and SLIK histone acetyltransferase activity. Proc Natl Acad Sci U S A 102(24):8478-82
3) Shukla A, et al.  (2006) SAGA-associated Sgf73p facilitates formation of the preinitiation complex assembly at the promoters either in a HAT-dependent or independent manner in vivo. Nucleic Acids Res 34(21):6225-32
4) Helmlinger D, et al.  (2004) Ataxin-7 is a subunit of GCN5 histone acetyltransferase-containing complexes. Hum Mol Genet 13(12):1257-65
5) Jordan PW, et al.  (2007) Novel roles for selected genes in meiotic DNA processing. PLoS Genet 3(12):e222
6) Lee KK, et al.  (2009) Yeast Sgf73/Ataxin-7 serves to anchor the deubiquitination module into both SAGA and Slik(SALSA) HAT complexes. Epigenetics Chromatin 2(1):2
7) Tous C, et al.  (2011) A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors. EMBO J 30(10):1953-64
8) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30