SGA1/YIL099W Summary Help

Standard Name SGA1 1
Systematic Name YIL099W
Feature Type ORF, Verified
Description Intracellular sporulation-specific glucoamylase; involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation (1, 2, 3, 4 and see Summary Paragraph)
Name Description Sporulation-specific GlycoAmylase 1
Chromosomal Location
ChrIX:178004 to 179653 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SGA1 GO evidence and references
  View Computational GO annotations for SGA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 9 genes
Resources
Pathways
Large-scale survey
null
Resources
8 total interaction(s) for 8 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1

Genetic Interactions
  • Negative Genetic: 5
  • Positive Genetic: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 549
Molecular Weight (Da) 61,462
Isoelectric Point (pI) 4.74
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:178004 to 179653 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1650 178004..179653 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001361
SUMMARY PARAGRAPH for SGA1

Glycogen is a branched polysaccharide of high molecular mass that is used as a storage carbohydrate. In S. cerevisiae, glycogen is typically catabolized to glucose-1-phosphate and glucose by the Gph1p glycogen phosphorylase and the Gdb1p debranching enzyme (5). However, during sporulation, glycogen is rapidly catabolized to glucose by the Sga1p glucoamylase (5).

SGA1 expression is inhibited by Rme1p during vegetative growth, and is induced by Ime1p in diploid cells during late sporulation (6). Co-localization experiments suggest that Sga1p may localize to the vacuole (2). The Sga1p amylo-(1,4-1,6)-glucosidase is capable of degrading glycogen, starch, maltotriose, and maltose into glucose, but exhibits maximum activity toward glycogen at pH 5.5 (5, 2). The role of glycogen degradation during sporulation is not fully understood, as approximately 90% of sga1 homozygous null mutants are able to produce viable spores (5).

Last updated: 2005-08-25 Contact SGD

References cited on this page View Complete Literature Guide for SGA1
1) Yamashita I and Fukui S  (1985) Transcriptional control of the sporulation-specific glucoamylase gene in the yeast Saccharomyces cerevisiae. Mol Cell Biol 5(11):3069-73
2) Pugh TA, et al.  (1989) Characterization and localization of the sporulation glucoamylase of Saccharomyces cerevisiae. Biochim Biophys Acta 994(3):200-9
3) Wang Z, et al.  (2001) Antagonistic controls of autophagy and glycogen accumulation by Snf1p, the yeast homolog of AMP-activated protein kinase, and the cyclin-dependent kinase Pho85p. Mol Cell Biol 21(17):5742-52
4) Clancy MJ, et al.  (1982) Developmental regulation of a sporulation-specific enzyme activity in Saccharomyces cerevisiae. Mol Cell Biol 2(2):171-8
5) Francois J and Parrou JL  (2001) Reserve carbohydrates metabolism in the yeast Saccharomyces cerevisiae. FEMS Microbiol Rev 25(1):125-45
6) Kihara K, et al.  (1991) Positive and negative elements upstream of the meiosis-specific glucoamylase gene in Saccharomyces cerevisiae. Mol Gen Genet 226(3):383-92