SFI1/YLL003W Summary Help

Standard Name SFI1 1
Systematic Name YLL003W
Feature Type ORF, Verified
Description Centrin (Cdc31p)-binding protein required for SPB duplication; localizes to the half-bridge of the spindle pole body (SPB); required for progression through G(2)-M transition; phosphorylated by Cdc28p-Clb2p and by Cdc5p; dephosphorylated by Cdc14p; has similarity to Xenopus laevis XCAP-C (1, 2, 3)
Name Description Suppressor of Fermentation Induced loss of stress resistance 1
Chromosomal Location
ChrXII:143201 to 146041 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SFI1 GO evidence and references
  View Computational GO annotations for SFI1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
conditional
null
overexpression
reduction of function
repressible
Large-scale survey
null
overexpression
reduction of function
Resources
57 total interaction(s) for 45 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-Western: 4
  • Biochemical Activity: 1
  • Protein-peptide: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 6
  • Negative Genetic: 16
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 13

Resources
Expression Summary
histogram
Resources
Length (a.a.) 946
Molecular Weight (Da) 112,978
Isoelectric Point (pI) 10.59
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:143201 to 146041 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2841 143201..146041 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003926
References cited on this page View Complete Literature Guide for SFI1
1) Ma P, et al.  (1999) Deletion of SFI1, a novel suppressor of partial Ras-cAMP pathway deficiency in the yeast Saccharomyces cerevisiae, causes G(2) arrest. Yeast 15(11):1097-109
2) Kilmartin JV  (2003) Sfi1p has conserved centrin-binding sites and an essential function in budding yeast spindle pole body duplication. J Cell Biol 162(7):1211-21
3) Elserafy M, et al.  (2014) Molecular Mechanisms that Restrict Yeast Centrosome Duplication to One Event per Cell Cycle. Curr Biol 24(13):1456-66