SET2/YJL168C Summary Help

Standard Name SET2 1
Systematic Name YJL168C
Alias EZL1
Feature Type ORF, Verified
Description Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36 (H3K36), which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain(CTD) of Rpo21p; H3K36me3 (trimethylation) requires Spt6p, proline 38 on H3, CTD of Rpo21p, Ctk1p, and C-terminal SRI domain of Ste2p; relocalizes to the cytosol in response to hypoxia (2, 3, 4, 5, 6, 7, 8)
Name Description SET domain-containing 9
Gene Product Alias KMT3 10
Chromosomal Location
ChrX:104428 to 102227 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All SET2 GO evidence and references
  View Computational GO annotations for SET2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
Large-scale survey
727 total interaction(s) for 391 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 49
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 17
  • Biochemical Activity: 2
  • Co-localization: 2
  • Protein-peptide: 31
  • Reconstituted Complex: 10
  • Two-hybrid: 28

Genetic Interactions
  • Dosage Growth Defect: 2
  • Negative Genetic: 289
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 2
  • Positive Genetic: 47
  • Synthetic Growth Defect: 144
  • Synthetic Lethality: 71
  • Synthetic Rescue: 22

Expression Summary
Length (a.a.) 733
Molecular Weight (Da) 84,461
Isoelectric Point (pI) 8.67
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrX:104428 to 102227 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2202 104428..102227 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003704
References cited on this page View Complete Literature Guide for SET2
1) Hesman, T. and M. Johnston  (1997) SET2p contains both SET and CXC domains and regulates GAL4 expression. Unpublished
2) Strahl BD, et al.  (2002) Set2 is a nucleosomal histone H3-selective methyltransferase that mediates transcriptional repression. Mol Cell Biol 22(5):1298-306
3) Xiao T, et al.  (2003) Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast. Genes Dev 17(5):654-63
4) Schaft D, et al.  (2003) The histone 3 lysine 36 methyltransferase, SET2, is involved in transcriptional elongation. Nucleic Acids Res 31(10):2475-82
5) Li B, et al.  (2003) The Set2 histone methyltransferase functions through the phosphorylated carboxyl-terminal domain of RNA polymerase II. J Biol Chem 278(11):8897-903
6) Youdell ML, et al.  (2008) Roles for Ctk1 and Spt6 in regulating the different methylation states of histone H3 lysine 36. Mol Cell Biol 28(16):4915-26
7) Venkatesh S, et al.  (2012) Set2 methylation of histone H3 lysine?36 suppresses histone exchange on transcribed genes. Nature 489(7416):452-5
8) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30
9) Nislow C, et al.  (1997) SET1, a yeast member of the trithorax family, functions in transcriptional silencing and diverse cellular processes. Mol Biol Cell 8(12):2421-36
10) Allis CD, et al.  (2007) New nomenclature for chromatin-modifying enzymes. Cell 131(4):633-6