SEC8/YPR055W Summary Help

Standard Name SEC8 1, 2
Systematic Name YPR055W
Feature Type ORF, Verified
Description Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress (3, 4, 5, 6)
Name Description SECretory 7
Chromosomal Location
ChrXVI:667676 to 670873 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SEC8 GO evidence and references
  View Computational GO annotations for SEC8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant phenotypes All SEC8 Phenotype evidence and references
Classical genetics
conditional
Large-scale survey
null
repressible
Resources
interactions All SEC8 Interaction evidence and references
146 total interaction(s) for 37 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 27
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 43
  • Co-fractionation: 4
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 4
Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 3
  • Dosage Rescue: 13
  • Positive Genetic: 1
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 39
  • Synthetic Rescue: 2
Resources
Expression Summary
histogram
Resources
Protein Information All SEC8 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:667676 to 670873 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..3198 667676..670873 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006259
References cited on this page View Complete Literature Guide for SEC8
1) Bowser R, et al.  (1992) Sec8p and Sec15p are components of a plasma membrane-associated 19.5S particle that may function downstream of Sec4p to control exocytosis. J Cell Biol 118(5):1041-56
2) Novick P, et al.  (1980) Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell 21(1):205-15
3) TerBush DR, et al.  (1996) The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae. EMBO J 15(23):6483-94
4) TerBush DR and Novick P  (1995) Sec6, Sec8, and Sec15 are components of a multisubunit complex which localizes to small bud tips in Saccharomyces cerevisiae. J Cell Biol 130(2):299-312
5) Reinke CA, et al.  (2004) Golgi inheritance in small buds of Saccharomyces cerevisiae is linked to endoplasmic reticulum inheritance. Proc Natl Acad Sci U S A 101(52):18018-23
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7) Novick P and Schekman R  (1979) Secretion and cell-surface growth are blocked in a temperature-sensitive mutant of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 76(4):1858-62