SEC3/YER008C Summary Help

Standard Name SEC3 1, 2
Systematic Name YER008C
Alias PSL1 3
Feature Type ORF, Verified
Description Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress (4, 5, 6, 7, 8, 9, 10, 11, 12)
Name Description SECretory 13
Chromosomal Location
ChrV:171818 to 167808 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 3 cM
Gene Ontology Annotations All SEC3 GO evidence and references
  View Computational GO annotations for SEC3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
reduction of function
219 total interaction(s) for 114 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 32
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 10
  • Biochemical Activity: 1
  • Co-crystal Structure: 2
  • Co-fractionation: 1
  • Co-purification: 1
  • Reconstituted Complex: 10
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 8
  • Dosage Lethality: 1
  • Dosage Rescue: 26
  • Negative Genetic: 52
  • Phenotypic Enhancement: 4
  • Positive Genetic: 5
  • Synthetic Growth Defect: 16
  • Synthetic Lethality: 38
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 1,336
Molecular Weight (Da) 154,694
Isoelectric Point (pI) 5.24
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrV:171818 to 167808 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 3 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..4011 171818..167808 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000810
References cited on this page View Complete Literature Guide for SEC3
1) Novick P, et al.  (1980) Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell 21(1):205-15
2) Fields, C. and Schekman, R.  (1985) Personal Communication, Mortimer Map Edition 9
3) Haarer BK, et al.  (1996) SEC3 mutations are synthetically lethal with profilin mutations and cause defects in diploid-specific bud-site selection. Genetics 144(2):495-510
4) Finger FP and Novick P  (1998) Spatial regulation of exocytosis: lessons from yeast. J Cell Biol 142(3):609-12
5) Finger FP, et al.  (1998) Sec3p is a spatial landmark for polarized secretion in budding yeast. Cell 92(4):559-71
6) TerBush DR, et al.  (1996) The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae. EMBO J 15(23):6483-94
7) TerBush DR and Novick P  (1995) Sec6, Sec8, and Sec15 are components of a multisubunit complex which localizes to small bud tips in Saccharomyces cerevisiae. J Cell Biol 130(2):299-312
8) Guo W, et al.  (2001) Spatial regulation of the exocyst complex by Rho1 GTPase. Nat Cell Biol 3(4):353-60
9) Wiederkehr A, et al.  (2003) Sec3p is needed for the spatial regulation of secretion and for the inheritance of the cortical endoplasmic reticulum. Mol Biol Cell 14(12):4770-82
10) He B, et al.  (2007) Exo70 interacts with phospholipids and mediates the targeting of the exocyst to the plasma membrane. EMBO J 26(18):4053-65
11) Hutagalung AH, et al.  (2009) An internal domain of Exo70p is required for actin-independent localization and mediates assembly of specific exocyst components. Mol Biol Cell 20(1):153-63
12) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
13) Novick P and Schekman R  (1979) Secretion and cell-surface growth are blocked in a temperature-sensitive mutant of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 76(4):1858-62