SEC17/YBL050W Summary Help

Standard Name SEC17 1
Systematic Name YBL050W
Alias RNS3 2
Feature Type ORF, Verified
Description Alpha-SNAP cochaperone; SNARE-complex adaptor for Sec18 (NSF) during the disassembly of postfusion cis-SNARE complexes; stimulates the ATPase activity of Sec18p; peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; similar to mammalian alpha-SNAP (3, 4, 5, 6, 7, 8, 9 and see Summary Paragraph)
Name Description SECretory 10
Chromosomal Location
ChrII:125125 to 126119 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SEC17 GO evidence and references
  View Computational GO annotations for SEC17
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 115 genes
Resources
Classical genetics
conditional
null
overexpression
repressible
Large-scale survey
conditional
null
overexpression
reduction of function
repressible
Resources
181 total interaction(s) for 125 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 45
  • Co-fractionation: 6
  • Co-purification: 4
  • Reconstituted Complex: 7

Genetic Interactions
  • Dosage Lethality: 3
  • Dosage Rescue: 3
  • Negative Genetic: 83
  • Positive Genetic: 7
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 292
Molecular Weight (Da) 32,802
Isoelectric Point (pI) 4.8
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:125125 to 126119 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..30 125125..125154 2011-02-03 1997-01-28
Intron 31..146 125155..125270 2011-02-03 1997-01-28
CDS 147..995 125271..126119 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000146
SUMMARY PARAGRAPH for SEC17

SEC17 and SEC18 act as SNARE chaperones during protein transport between organelles (such as ER to Golgi transport), transport to and from the plasma membrane (such as protein secretion), and fusion between organelles (such as homotypic vacuole fusion) (11, 12). Sec18p is an AAA-ATPase whose activity is stimulated by Sec17p (3). Sec18p is the yeast homolog of the mammalian NSF and Sec17p is the yeast homolog of the mammalian alpha-SNAP (13, 4).

During ER to Golgi transport, Sec17p and Sec18p bind SNARE complexes that formed after the ER-derived vesicle has docked with the Golgi membrane and are required for membrane fusion (5, 14, 15). In contrast, during homotypic vacuole fusion, Sec17p and Sec18p are required prior to docking and membrane fusion (7). Sec17p and Sec18p bind SNARE complexes that have accumulated on vacuole membranes from previous fusion events. Then the ATPase activity of Sec18p drives disassembly of these SNARE complexes and primes them for another round of vacuolar fusion (6). Based on Sec17p and Sec18p function during yeast homotypic vacuole fusion as well as studies in mammalian systems of other membrane fusion events, Sec17p and Sec18p are proposed to facilitate the recycling of SNARE proteins during all membrane fusion events (16).

Last updated: 2010-05-12 Contact SGD

References cited on this page View Complete Literature Guide for SEC17
1) Novick P, et al.  (1980) Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell 21(1):205-15
2) Ishikawa T, et al.  (2005) Isolation of Saccharomyces cerevisiae RNase T1 hypersensitive (rns) mutants and genetic analysis of the RNS1/DSL1 gene. J Gen Appl Microbiol 51(1):73-82
3) Steel GJ, et al.  (1999) Biochemical analysis of the Saccharomyces cerevisiae SEC18 gene product: implications for the molecular mechanism of membrane fusion. Biochemistry 38(24):7764-72
4) Griff IC, et al.  (1992) The yeast SEC17 gene product is functionally equivalent to mammalian alpha-SNAP protein. J Biol Chem 267(17):12106-15
5) Sogaard M, et al.  (1994) A rab protein is required for the assembly of SNARE complexes in the docking of transport vesicles. Cell 78(6):937-48
6) Mayer A, et al.  (1996) Sec18p (NSF)-driven release of Sec17p (alpha-SNAP) can precede docking and fusion of yeast vacuoles. Cell 85(1):83-94
7) Ungermann C, et al.  (1998) A vacuolar v-t-SNARE complex, the predominant form in vivo and on isolated vacuoles, is disassembled and activated for docking and fusion. J Cell Biol 140(1):61-9
8) Ishihara N, et al.  (2001) Autophagosome requires specific early Sec proteins for its formation and NSF/SNARE for vacuolar fusion. Mol Biol Cell 12(11):3690-702
9) Mima J, et al.  (2008) Reconstituted membrane fusion requires regulatory lipids, SNAREs and synergistic SNARE chaperones. EMBO J 27(15):2031-42
10) Novick P and Schekman R  (1979) Secretion and cell-surface growth are blocked in a temperature-sensitive mutant of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 76(4):1858-62
11) Bonifacino JS and Glick BS  (2004) The mechanisms of vesicle budding and fusion. Cell 116(2):153-66
12) Ostrowicz CW, et al.  (2008) Yeast vacuole fusion: a model system for eukaryotic endomembrane dynamics. Autophagy 4(1):5-19
13) Wilson DW, et al.  (1989) A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast. Nature 339(6223):355-9
14) Barlowe C  (1997) Coupled ER to Golgi transport reconstituted with purified cytosolic proteins. J Cell Biol 139(5):1097-108
15) Muniz M, et al.  (2001) Protein sorting upon exit from the endoplasmic reticulum. Cell 104(2):313-20
16) Hong W  (2005) SNAREs and traffic. Biochim Biophys Acta 1744(2):120-44