SEC11/YIR022W Summary Help

Standard Name SEC11 1
Systematic Name YIR022W
Feature Type ORF, Verified
Description 18kDa catalytic subunit of the Signal Peptidase Complex (SPC); the Signal Peptidase Complex cleaves the signal sequence of proteins targeted to the endoplasmic reticulum; other members are Spc1p, Spc2p, Spc3p, and Sec11p (2, 3 and see Summary Paragraph)
Name Description SECretory 4
Chromosomal Location
ChrIX:398733 to 399236 | ORF Map | GBrowse
Genetic position: 22 cM
Gene Ontology Annotations All SEC11 GO evidence and references
  View Computational GO annotations for SEC11
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
Large-scale survey
reduction of function
133 total interaction(s) for 107 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 11
  • Affinity Capture-RNA: 5
  • Co-purification: 1
  • PCA: 7

Genetic Interactions
  • Dosage Rescue: 5
  • Negative Genetic: 75
  • Phenotypic Enhancement: 1
  • Positive Genetic: 20
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 6

Expression Summary
Length (a.a.) 167
Molecular Weight (Da) 18,762
Isoelectric Point (pI) 9.96
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIX:398733 to 399236 | ORF Map | GBrowse
Genetic position: 22 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..504 398733..399236 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001461

SEC11 encodes the catalytic subunit of the signal peptidase complex (SPC), which cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER) (2, 3). Signal peptide cleavage occurs concomitantly with translocation through the translocon pore into the ER. In yeast, translocation can occur cotranslationally or posttranslationally, whereas in mammals translocation is always cotranslational. The process of protein translocation into the ER is reviewed in references 5 and 6.

The yeast SPC comprises four proteins, Spc1p, Spc2p, Spc3p, and Sec11p (3, 7). SEC11 is essential for viability and for signal peptidase activity (2). SEC11 interacts genetically with the genes encoding the other SPC subunits (8, 9, 10), and Sec11p can be coimmunoprecipitated with Spc3p (11).

Sec11p is homologous to the mammalian signal peptidase subunits SPC18 and SPC21 (12), and shows some sequence similarity the catalytic site of the Bacillus subtilis leader peptidase SipW (11). Indeed, Sec11p has signal peptidase catalytic activity, although the phenotypes of mutations in the putative catalytic site of Sec11p suggest that the catalytic mechanism of Sec11p differs from that of SipW (11).

Last updated: 2000-12-08 Contact SGD

References cited on this page View Complete Literature Guide for SEC11
1) Novick P, et al.  (1980) Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell 21(1):205-15
2) Bohni PC, et al.  (1988) SEC11 is required for signal peptide processing and yeast cell growth. J Cell Biol 106(4):1035-42
3) YaDeau JT, et al.  (1991) Yeast signal peptidase contains a glycoprotein and the Sec11 gene product. Proc Natl Acad Sci U S A 88(2):517-21
4) Novick P and Schekman R  (1979) Secretion and cell-surface growth are blocked in a temperature-sensitive mutant of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 76(4):1858-62
5) Rapoport TA, et al.  (1996) Protein transport across the eukaryotic endoplasmic reticulum and bacterial inner membranes. Annu Rev Biochem 65:271-303
6) Johnson AE and van Waes MA  (1999) The translocon: a dynamic gateway at the ER membrane. Annu Rev Cell Dev Biol 15:799-842
7) Antonin W, et al.  (2000) Interactions between Spc2p and other components of the endoplasmic reticulum translocation sites of the yeast Saccharomyces cerevisiae. J Biol Chem 275(44):34068-72
8) Mullins C, et al.  (1996) Structurally related Spc1p and Spc2p of yeast signal peptidase complex are functionally distinct. J Biol Chem 271(46):29094-9
9) Fang H, et al.  (1996) The homologue of mammalian SPC12 is important for efficient signal peptidase activity in Saccharomyces cerevisiae. J Biol Chem 271(28):16460-5
10) Fang H, et al.  (1997) In addition to SEC11, a newly identified gene, SPC3, is essential for signal peptidase activity in the yeast endoplasmic reticulum. J Biol Chem 272(20):13152-8
11) VanValkenburgh C, et al.  (1999) The catalytic mechanism of endoplasmic reticulum signal peptidase appears to be distinct from most eubacterial signal peptidases. J Biol Chem 274(17):11519-25
12) Shelness GS and Blobel G  (1990) Two subunits of the canine signal peptidase complex are homologous to yeast SEC11 protein. J Biol Chem 265(16):9512-9