SDP1/YIL113W Summary Help

Standard Name SDP1 1
Systematic Name YIL113W
Feature Type ORF, Verified
Description Stress-inducible dual-specificity MAP kinase phosphatase; negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock; SDP1 has a paralog, MSG5, that arose from the whole genome duplication (1, 2, 3 and see Summary Paragraph)
Name Description Stress-inducible Dual specificity Phosphatase 1
Chromosomal Location
ChrIX:150559 to 151188 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SDP1 GO evidence and references
  View Computational GO annotations for SDP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 7 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
27 total interaction(s) for 18 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-Western: 2
  • Biochemical Activity: 2
  • PCA: 2
  • Reconstituted Complex: 2
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 1
  • Negative Genetic: 6
  • Phenotypic Suppression: 1
  • Positive Genetic: 3
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 209
Molecular Weight (Da) 23,885
Isoelectric Point (pI) 7.99
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:150559 to 151188 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..630 150559..151188 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001375
SUMMARY PARAGRAPH for SDP1

SDP1 encodes a protein-tyrosine phosphatase with sequence similarity to mammalian dual-specificity MAP kinase phosphatases (MKPs) (4). Sdp1 is a stress-inducible enzyme that specifically interacts with and dephosphorylates the stress-activated Slt2p MAP kinase following heat shock (4, 1). Slt2 is also activated in response to oxidative stress, conditions under which mammalian MKPs are reversibly inactivated by oxidation of the catalytic cysteine residue. However, Sdp1p employs a novel catalytic mechanism that may allow it to remain active under oxidative conditions (2). When oxidized, both Sdp1p and its paralog Msg5p form a disulfide bond between two additional cysteine residues. This disulfide is required for selective recognition of a tyrosine phosphorylated MAPK substrate and for optimal catalytic activity. Disulfide bond formation appears to be specific to the budding yeast MPKs, which are significantly diverged from metazoan and plant MPKs (5), and is not found in dual-specificity MKPs from filamentous fungi or fission yeasts (2).

Under normal growth conditions, Sdp1p is diffusely localized in both the nucleus and cytoplasm, but translocates to punctate spots throughout the cell after heat shock. SDP1 transcription is induced by several stress conditions in an Msn2p/Msn4p-dependent manner, but is independent of the Rlm1p transcription factor, a downstream target activated by Slt2p (1).

Sdp1p specifically interacts with the stress-activated Slt2p MAP kinase, and expression of an inactive mutant of Sdp1p causes the accumulation of phosphorylated Slt2p, indicating that Slt2p is a physiological target of the Sdp1p phosphatase (2). Heat shock-induced Slt2p phosphorylation levels are elevated in the absence of Sdp1p (1) and Sdp1p is required for the dephosphorylation of Slt2p during oxidative conditions (4).

Regarding usage of the SDP1 name

The SDP1 name has also been used for a recessive extragenic suppressor of temperature-sensitive mutations in the POL3 gene encoding the catalytic subunit of DNA polymerase delta (6). However, as of July 2007, the gene corresponding to this POL3 suppressor mutation has not yet been identified (7).

Last updated: 2005-04-05 Contact SGD

References cited on this page View Complete Literature Guide for SDP1
1) Hahn JS and Thiele DJ  (2002) Regulation of the Saccharomyces cerevisiae Slt2 kinase pathway by the stress-inducible Sdp1 dual specificity phosphatase. J Biol Chem 277(24):21278-84
2) Collister M, et al.  (2002) YIL113w encodes a functional dual-specificity protein phosphatase which specifically interacts with and inactivates the Slt2/Mpk1p MAP kinase in S. cerevisiae. FEBS Lett 527(1-3):186-92
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Fox GC, et al.  (2007) Redox-mediated substrate recognition by Sdp1 defines a new group of tyrosine phosphatases. Nature 447(7143):487-92
5) Nordle AK, et al.  (2007) Functional assignment of MAPK phosphatase domains. Proteins 69(1):19-31
6) Giot L, et al.  (1995) Suppressors of thermosensitive mutations in the DNA polymerase delta gene of Saccharomyces cerevisiae. Mol Gen Genet 246(2):212-22
7) Chanet R  (2007) Personal communication: Multiple uses of the SDP1 name