SAW1/YAL027W Summary Help

Standard Name SAW1 1
Systematic Name YAL027W
Feature Type ORF, Verified
Description 5'- and 3'-flap DNA binding protein; recruits Rad1p-Rad10p to single-strand annealing intermediates with 3' non-homologous tails for removal during double-strand break repair; complexes with Rad1p-Rad10p and stimulates its endonuclease activity; green fluorescent protein (GFP)-fusion protein localizes to the nucleus (1, 2, 3, 4)
Name Description Single-strand Annealing Weakened 1
Chromosomal Location
ChrI:94687 to 95472 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SAW1 GO evidence and references
  View Computational GO annotations for SAW1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
69 total interaction(s) for 48 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 34
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 6
  • Co-localization: 1
  • Reconstituted Complex: 9
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 11
  • Phenotypic Enhancement: 2
  • Positive Genetic: 1
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 261
Molecular Weight (Da) 29,769
Isoelectric Point (pI) 10.12
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrI:94687 to 95472 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..786 94687..95472 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000025
References cited on this page View Complete Literature Guide for SAW1
1) Li F, et al.  (2008) Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates. Mol Cell 30(3):325-35
2) Giaever G, et al.  (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91
3) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
4) Li F, et al.  (2013) Role of Saw1 in Rad1/Rad10 complex assembly at recombination intermediates in budding yeast. EMBO J 32(3):461-72