SAN1/YDR143C Summary Help

Standard Name SAN1 1
Systematic Name YDR143C
Feature Type ORF, Verified
Description Ubiquitin-protein ligase; involved in proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition; prefers a window of exposed hydrophobicity that causes a particular level of protein insolubility, suggesting that San1p evolved to target highly aggregation-prone proteins (2, 3, 4, 5, 6)
Name Description Sir Antagonist 1
Chromosomal Location
ChrIV:743874 to 742042 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SAN1 GO evidence and references
  View Computational GO annotations for SAN1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
124 total interaction(s) for 92 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 40
  • Affinity Capture-RNA: 2
  • Co-purification: 1
  • Protein-RNA: 1
  • Two-hybrid: 28

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 1
  • Negative Genetic: 14
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 8
  • Positive Genetic: 6
  • Synthetic Growth Defect: 3
  • Synthetic Rescue: 10

Resources
Expression Summary
histogram
Resources
Length (a.a.) 610
Molecular Weight (Da) 65,618
Isoelectric Point (pI) 4.93
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:743874 to 742042 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1833 743874..742042 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002550
References cited on this page View Complete Literature Guide for SAN1
1) Schnell R, et al.  (1989) Genetic and molecular characterization of suppressors of SIR4 mutations in Saccharomyces cerevisiae. Genetics 122(1):29-46
2) Dasgupta A, et al.  (2004) Sir Antagonist 1 (San1) is a ubiquitin ligase. J Biol Chem 279(26):26830-8
3) Gardner RG, et al.  (2005) Degradation-mediated protein quality control in the nucleus. Cell 120(6):803-15
4) Rosenbaum JC, et al.  (2011) Disorder targets misorder in nuclear quality control degradation: a disordered ubiquitin ligase directly recognizes its misfolded substrates. Mol Cell 41(1):93-106
5) Fredrickson EK, et al.  (2011) Exposed hydrophobicity is a key determinant of nuclear quality control degradation. Mol Biol Cell 22(13):2384-95
6) Fredrickson EK, et al.  (2013) Substrate recognition in nuclear protein quality control degradation is governed by exposed hydrophobicity that correlates with aggregation and insolubility. J Biol Chem 288(9):6130-9