SAK1/YER129W Summary Help

Standard Name SAK1 1, 2
Systematic Name YER129W
Alias PAK1 3
Feature Type ORF, Verified
Description Upstream serine/threonine kinase for the SNF1 complex; plays a role in pseudohyphal groth; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome; SAK1 has a paralog, TOS3, that arose from the whole genome duplication (1, 3, 4, 5, 6, 7, 8)
Name Description Snf1 Activating Kinase 1
Chromosomal Location
ChrV:417281 to 420709 | ORF Map | GBrowse
Gene Ontology Annotations All SAK1 GO evidence and references
  View Computational GO annotations for SAK1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Classical genetics
Large-scale survey
108 total interaction(s) for 67 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 29
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 11
  • Biochemical Activity: 8
  • Co-fractionation: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 2
  • Negative Genetic: 29
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 3
  • Positive Genetic: 3
  • Synthetic Growth Defect: 12
  • Synthetic Lethality: 3
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 1,142
Molecular Weight (Da) 126,871
Isoelectric Point (pI) 9.02
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrV:417281 to 420709 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3429 417281..420709 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000931
References cited on this page View Complete Literature Guide for SAK1
1) Elbing K, et al.  (2006) Purification and characterization of the three Snf1-activating kinases of Saccharomyces cerevisiae. Biochem J 393(Pt 3):797-805
2) Rubenstein EM, et al.  (2006) Regulatory domains of Snf1-activating kinases determine pathway specificity. Eukaryot Cell 5(4):620-7
3) Hovland PG, et al.  (1997) Overexpression of the protein kinase Pak1 suppresses yeast DNA polymerase mutations. Mol Gen Genet 256(1):45-53
4) Nath N, et al.  (2003) Yeast Pak1 kinase associates with and activates Snf1. Mol Cell Biol 23(11):3909-17
5) Sutherland CM, et al.  (2003) Elm1p is one of three upstream kinases for the Saccharomyces cerevisiae SNF1 complex. Curr Biol 13(15):1299-305
6) Hong SP, et al.  (2003) Activation of yeast Snf1 and mammalian AMP-activated protein kinase by upstream kinases. Proc Natl Acad Sci U S A 100(15):8839-43
7) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
8) Orlova M, et al.  (2010) Roles of the Snf1-activating kinases during nitrogen limitation and pseudohyphal differentiation in Saccharomyces cerevisiae. Eukaryot Cell 9(1):208-14