Standard Name	SAE2 1
Systematic Name	YGL175C
Alias	COM1 2
Feature Type	ORF, Verified
Description	Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents (1, 2, 3, 4, 5)
Name Description	Sporulation in the Absence of spo Eleven 1

Chromosomal Location	ChrVII:174322 to 173285 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for SAE2
Molecular Function
Manually curated	double-stranded DNA binding (IDA, IMP) single-stranded DNA specific endodeoxyribonuclease activity (IDA, IMP)
Biological Process
Manually curated	DNA catabolic process, endonucleolytic (IDA, IMP) DNA double-strand break processing (IGI) gene conversion at mating-type locus, DNA double-strand break processing (IMP) meiotic DNA double-strand break formation (IGI) meiotic DNA double-strand break processing (IGI, IMP)
Cellular Component
High-throughput	cytoplasm (IDA) nucleus (IDA)

Classical genetics
null	gamma ray resistance: decreased resistance to chromium(6+): decreased resistance to chromium(6+): normal transposable element transposition: increased
reduction of function	mitotic recombination: decreased sporulation: absent
Large-scale survey
null	UV resistance: decreased auxotrophy competitive fitness: decreased gamma ray resistance: decreased mitotic recombination: increased Rad52-YFP distribution: increased resistance to cisplatin: decreased resistance to hydroxyurea: decreased resistance to doxorubicin: decreased resistance to benzo[a]pyrene: decreased resistance to benzo[a]pyrene: increased resistance to amitrole: decreased resistance to cycloheximide: decreased resistance to caffeine: increased resistance to fenpropimorph: increased resistance to fluconazole: increased resistance to tunicamycin: increased resistance to sodium selenide: decreased resistance to methyl methanesulfonate: decreased resistance to camptothecin: decreased sporulation: decreased sporulation efficiency: decreased transposable element transposition: increased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	131 total interaction(s) for 72 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-RNA: 1 Affinity Capture-Western: 3 Far Western: 1 Reconstituted Complex: 3 Two-hybrid: 8
Genetic Interactions	Dosage Growth Defect: 6 Dosage Lethality: 3 Dosage Rescue: 2 Negative Genetic: 44 Phenotypic Enhancement: 13 Phenotypic Suppression: 8 Positive Genetic: 1 Synthetic Growth Defect: 17 Synthetic Lethality: 16 Synthetic Rescue: 3
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVII:174322 to 173285 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1038 174322..173285 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000003143