SAE2/YGL175C Summary

SAE2 BASIC INFORMATION

Standard Name	SAE2 1
Systematic Name	YGL175C
Alias	COM1 2
Feature Type	ORF, Verified
Description	Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents (1, 2, 3, 4, 5)
Name Description	Sporulation in the Absence of spo Eleven 1

GO Annotations	All SAE2 GO evidence and references
	View Computational GO annotations for SAE2
Molecular Function
Manually curated	double-stranded DNA binding (IDA, IMP) single-stranded DNA specific endodeoxyribonuclease activity (IDA, IMP)
Biological Process
Manually curated	DNA catabolic process, endonucleolytic (IDA, IMP) DNA double-strand break processing (IGI) gene conversion at mating-type locus, DNA double-strand break processing (IMP) meiotic DNA double-strand break processing (IMP)
Cellular Component
High-throughput	cytoplasm (IDA) nucleus (IDA)

Mutant Phenotype	All SAE2 Phenotype details and references
Classical genetics
null	resistance to chromium(6+): decreased resistance to chromium(6+): normal transposable element transposition: increased
reduction of function	mitotic recombination: decreased sporulation: absent
Large-scale survey
null	mitotic recombination: increased Rad52-YFP distribution: increased resistance to hydroxyurea: decreased resistance to methyl methanesulfonate: decreased resistance to cisplatin: decreased resistance to doxorubicin: decreased sporulation: decreased transposable element transposition: increased viable

Interactions	SAE2 All interactions details and references
	63 total interaction(s) for 41 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 1 Affinity Capture-Western: 2 Reconstituted Complex: 1 Two-hybrid: 7
Genetic Interactions	Phenotypic Enhancement: 24 Phenotypic Suppression: 2 Synthetic Growth Defect: 11 Synthetic Lethality: 13 Synthetic Rescue: 1

Sequence Information

ChrVII:174328 to 173291 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2004-07-20 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1038	174328..173291	2004-07-20	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000003143

SAE2 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for SAE2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SAE2]

1)	McKee AH and Kleckner N (1997) A general method for identifying recessive diploid-specific mutations in Saccharomyces cerevisiae, its application to the isolation of mutants blocked at intermediate stages of meiotic prophase and characterization of a new gene SAE2. Genetics 146(3):797-816
2)	Prinz S, et al. (1997) Isolation of COM1, a new gene required to complete meiotic double-strand break-induced recombination in Saccharomyces cerevisiae. Genetics 146(3):781-95
3)	Rattray AJ, et al. (2001) Fidelity of mitotic double-strand-break repair in Saccharomyces cerevisiae: a role for SAE2/COM1. Genetics 158(1):109-22
4)	Baroni E, et al. (2004) The functions of budding yeast Sae2 in the DNA damage response require Mec1- and Tel1-dependent phosphorylation. Mol Cell Biol 24(10):4151-65
5)	Lengsfeld BM, et al. (2007) Sae2 Is an Endonuclease that Processes Hairpin DNA Cooperatively with the Mre11/Rad50/Xrs2 Complex. Mol Cell 28(4):638-51

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