DID2/YKR035W-A Summary Help

Standard Name DID2 1
Systematic Name YKR035W-A
Alias FTI1 2 , CHM1 3 , VPS46 4 , VPL30 4
Feature Type ORF, Verified
Description Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors (1, 5, 6)
Name Description Doa4-Independent Degradation 1
Chromosomal Location
ChrXI:507939 to 508553 | ORF Map | GBrowse
Gene Ontology Annotations All DID2 GO evidence and references
  View Computational GO annotations for DID2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 8 genes
Classical genetics
reduction of function
Large-scale survey
114 total interaction(s) for 84 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-Western: 11
  • Co-crystal Structure: 1
  • Co-localization: 1
  • Far Western: 1
  • PCA: 4
  • Reconstituted Complex: 6
  • Two-hybrid: 28

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 34
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 2
  • Positive Genetic: 2
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 4
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 204
Molecular Weight (Da) 23,091
Isoelectric Point (pI) 6.87
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:507939 to 508553 | ORF Map | GBrowse
This feature is contained within: OPI8
Last Update Coordinates: 2011-02-03 | Sequence: 1999-07-17
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..615 507939..508553 2011-02-03 1999-07-17
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006435
References cited on this page View Complete Literature Guide for DID2
1) Amerik AY, et al.  (2000) The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell 11(10):3365-80
2) ter Schure EG, et al.  (2000) The role of ammonia metabolism in nitrogen catabolite repression in Saccharomyces cerevisiae. FEMS Microbiol Rev 24(1):67-83
3) Howard TL, et al.  (2001) CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins. J Cell Sci 114(Pt 13):2395-404
4) Raymond CK, et al.  (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
5) Nickerson DP, et al.  (2006) Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes. J Cell Biol 175(5):715-20
6) Rue SM, et al.  (2008) Novel ist1-did2 complex functions at a late step in multivesicular body sorting. Mol Biol Cell 19(2):475-84