CHL1/YPL008W Summary Help

Standard Name CHL1 1
Systematic Name YPL008W
Alias CTF1 2 , LPA9 , MCM12 3
Feature Type ORF, Verified
Description Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome (4, 5, 6, 7, 8)
Name Description CHromosome Loss 9
Chromosomal Location
ChrXVI:539385 to 541970 | ORF Map | GBrowse
Genetic position: -6 cM
Gene Ontology Annotations All CHL1 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Classical genetics
Large-scale survey
412 total interaction(s) for 249 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 7
  • Reconstituted Complex: 1
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 11
  • Dosage Rescue: 1
  • Negative Genetic: 177
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 1
  • Positive Genetic: 10
  • Synthetic Growth Defect: 52
  • Synthetic Lethality: 135

Expression Summary
Length (a.a.) 861
Molecular Weight (Da) 98,805
Isoelectric Point (pI) 7.91
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:539385 to 541970 | ORF Map | GBrowse
Genetic position: -6 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2586 539385..541970 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005929
References cited on this page View Complete Literature Guide for CHL1
1) Gerring SL, et al.  (1990) The CHL 1 (CTF 1) gene product of Saccharomyces cerevisiae is important for chromosome transmission and normal cell cycle progression in G2/M. EMBO J 9(13):4347-58
2) Spencer F, et al.  (1990) Mitotic chromosome transmission fidelity mutants in Saccharomyces cerevisiae. Genetics 124(2):237-49
3) Laha S, et al.  (2006) The budding yeast protein Chl1p is required to preserve genome integrity upon DNA damage in S-phase. Nucleic Acids Res 34(20):5880-91
4) Skibbens RV  (2004) Chl1p, a DNA helicase-like protein in budding yeast, functions in sister-chromatid cohesion. Genetics 166(1):33-42
5) Das SP and Sinha P  (2005) The budding yeast protein Chl1p has a role in transcriptional silencing, rDNA recombination, and aging. Biochem Biophys Res Commun 337(1):167-72
6) Wu Y, et al.  (2009) Welcome the family of FANCJ-like helicases to the block of genome stability maintenance proteins. Cell Mol Life Sci 66(7):1209-22
7) Wu Y, et al.  (2012) Biochemical characterization of Warsaw breakage syndrome helicase. J Biol Chem 287(2):1007-21
8) Ward TA, et al.  (2012) Components of a fanconi-like pathway control pso2-independent DNA interstrand crosslink repair in yeast. PLoS Genet 8(8):e1002884
9) Liras P, et al.  (1978) Characterization of a mutation in yeast causing nonrandom chromosome loss during mitosis. Genetics 88(4 Pt 1):651-71