CSE2/YNR010W Summary Help

Standard Name CSE2 1
Systematic Name YNR010W
Alias MED9
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; component of the Middle domain of mediator; required for regulation of RNA polymerase II activity; relocalizes to the cytosol in response to hypoxia (2, 3, 4, 5)
Name Description Chromosome SEgregation 1
Chromosomal Location
ChrXIV:643744 to 644193 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CSE2 GO evidence and references
  View Computational GO annotations for CSE2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 23 genes
Regulators 3 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
Resources
820 total interaction(s) for 477 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 59
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 56
  • Biochemical Activity: 1
  • Co-purification: 2
  • Reconstituted Complex: 4
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 537
  • Phenotypic Suppression: 2
  • Positive Genetic: 104
  • Synthetic Growth Defect: 25
  • Synthetic Lethality: 15
  • Synthetic Rescue: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 149
Molecular Weight (Da) 17,376
Isoelectric Point (pI) 6.36
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:643744 to 644193 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..450 643744..644193 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005293
References cited on this page View Complete Literature Guide for CSE2
1) Xiao Z, et al.  (1993) CSE1 and CSE2, two new genes required for accurate mitotic chromosome segregation in Saccharomyces cerevisiae. Mol Cell Biol 13(8):4691-702
2) Gustafsson CM, et al.  (1998) Identification of new mediator subunits in the RNA polymerase II holoenzyme from Saccharomyces cerevisiae. J Biol Chem 273(47):30851-4
3) Kang JS, et al.  (2001) The structural and functional organization of the yeast mediator complex. J Biol Chem 276(45):42003-10
4) Guglielmi B, et al.  (2004) A high resolution protein interaction map of the yeast Mediator complex. Nucleic Acids Res 32(18):5379-91
5) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30