NPR1/YNL183C Summary Help

Standard Name NPR1 1
Systematic Name YNL183C
Feature Type ORF, Verified
Description Protein kinase; stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex; NPR1 has a paralog, PRR2, that arose from the whole genome duplication (2, 3, 4, 5, 6)
Name Description Nitrogen Permease Reactivator 1
Chromosomal Location
ChrXIV:295509 to 293137 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NPR1 GO evidence and references
  View Computational GO annotations for NPR1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
unspecified
Resources
151 total interaction(s) for 131 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 20
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 6
  • Biochemical Activity: 6
  • Co-localization: 1
  • PCA: 1
  • Reconstituted Complex: 16
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 6
  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 71
  • Phenotypic Suppression: 3
  • Positive Genetic: 6
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 3
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 790
Molecular Weight (Da) 85,989
Isoelectric Point (pI) 8.4
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:295509 to 293137 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2373 295509..293137 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005127
References cited on this page View Complete Literature Guide for NPR1
1) Vandenbol M, et al.  (1987) Isolation of the NPR1 gene responsible for the reactivation of ammonia-sensitive amino-acid permeases in Saccharomyces cerevisiae. RNA analysis and gene dosage effects. Eur J Biochem 164(3):607-12
2) Schmidt A, et al.  (1998) The TOR nutrient signalling pathway phosphorylates NPR1 and inhibits turnover of the tryptophan permease. EMBO J 17(23):6924-31
3) De Craene JO, et al.  (2001) The Npr1 kinase controls biosynthetic and endocytic sorting of the yeast Gap1 permease. J Biol Chem 276(47):43939-48
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) O'Donnell AF, et al.  (2010) {alpha}-Arrestins Aly1 and Aly2 Regulate Intracellular Trafficking in Response to Nutrient Signaling. Mol Biol Cell 21(20):3552-66
6) Macgurn JA, et al.  (2011) TORC1 regulates endocytosis via Npr1-mediated phosphoinhibition of a ubiquitin ligase adaptor. Cell 147(5):1104-17