GAD1/YMR250W Summary Help

Standard Name GAD1 1
Systematic Name YMR250W
Feature Type ORF, Verified
Description Glutamate decarboxylase; converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress (1 and see Summary Paragraph)
Name Description GlutAmate Decarboxylase 1
Chromosomal Location
ChrXIII:770801 to 772558 | ORF Map | GBrowse
Gene Ontology Annotations All GAD1 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 18 genes
Classical genetics
Large-scale survey
35 total interaction(s) for 26 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Biochemical Activity: 2
  • PCA: 2
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 8
  • Phenotypic Enhancement: 3
  • Positive Genetic: 5
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 3

Expression Summary
Length (a.a.) 585
Molecular Weight (Da) 65,989
Isoelectric Point (pI) 6.61
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:770801 to 772558 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1758 770801..772558 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004862

About glutamate degradation

In S. cerevisiae, the main pathway for glutamate degradation is catalyzed by the glutamate dehydrogenase encoded by GDH2 (2). However, glutamate can also by degraded into gamma-aminobutyrate (GABA) by the glutamate decarboxylase Gad1p and then converted into succinate by the enzymes encoded by UGA1 and UGA2 (1). Glutamate degradation by this pathway and expression of its genes have been shown to be important for oxidative stress tolerance. Conditions of oxidative stress elevate the transcript levels of GAD1 and UGA2 (1). UGA1 and UGA2 expression is also upregulated in the presence of GABA which is mediated by the transcriptional activators Uga3p and Uga35p/Dal81p (3), 4). These transcription factors bind to upstream activation sites in the promoters of GABA-regulated genes known as the UAS-GABA (5, 4). Regulation of Gad1p is suggested to be linked to calcium levels as the protein is able to bind calmodulin (1). S. cerevisiae cells in which this pathway is blocked are more sensitive to oxidative stress and can no longer grow on GABA as their sole nitrogen source (1, 3).

Last updated: 2007-10-03 Contact SGD

References cited on this page View Complete Literature Guide for GAD1
1) Coleman ST, et al.  (2001) Expression of a glutamate decarboxylase homologue is required for normal oxidative stress tolerance in Saccharomyces cerevisiae. J Biol Chem 276(1):244-50
2) Miller SM and Magasanik B  (1990) Role of NAD-linked glutamate dehydrogenase in nitrogen metabolism in Saccharomyces cerevisiae. J Bacteriol 172(9):4927-35
3) Ramos F, et al.  (1985) Mutations affecting the enzymes involved in the utilization of 4-aminobutyric acid as nitrogen source by the yeast Saccharomyces cerevisiae. Eur J Biochem 149(2):401-4
4) Vissers S, et al.  (1989) Positive and negative regulatory elements control the expression of the UGA4 gene coding for the inducible 4-aminobutyric-acid-specific permease in Saccharomyces cerevisiae. Eur J Biochem 181(2):357-61
5) Idicula AM, et al.  (2002) Binding and activation by the zinc cluster transcription factors of Saccharomyces cerevisiae. Redefining the UASGABA and its interaction with Uga3p. J Biol Chem 277(48):45977-83