CAC2/YML102W Summary Help

Standard Name CAC2 1
Systematic Name YML102W
Feature Type ORF, Verified
Description Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure, deactivation of the DNA damage checkpoint after DNA repair, chromatin dynamics during transcription; and repression of divergent transcription; relocalizes to the cytosol in response to hypoxia (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
Name Description Chromatin Assembly Complex 1
Chromosomal Location
ChrXIII:68294 to 69700 | ORF Map | GBrowse
Gene Ontology Annotations All CAC2 GO evidence and references
  View Computational GO annotations for CAC2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
Large-scale survey
219 total interaction(s) for 118 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 19
  • Co-purification: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Growth Defect: 2
  • Negative Genetic: 89
  • Phenotypic Enhancement: 18
  • Phenotypic Suppression: 1
  • Positive Genetic: 21
  • Synthetic Growth Defect: 33
  • Synthetic Lethality: 6
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 468
Molecular Weight (Da) 51,252
Isoelectric Point (pI) 6.3
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:68294 to 69700 | ORF Map | GBrowse
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1407 68294..69700 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004570
References cited on this page View Complete Literature Guide for CAC2
1) Kaufman PD, et al.  (1997) Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I. Genes Dev 11(3):345-57
2) Enomoto S and Berman J  (1998) Chromatin assembly factor I contributes to the maintenance, but not the re-establishment, of silencing at the yeast silent mating loci. Genes Dev 12(2):219-32
3) Game JC and Kaufman PD  (1999) Role of Saccharomyces cerevisiae chromatin assembly factor-I in repair of ultraviolet radiation damage in vivo. Genetics 151(2):485-97
4) Smith JS, et al.  (1999) A genetic screen for ribosomal DNA silencing defects identifies multiple DNA replication and chromatin-modulating factors. Mol Cell Biol 19(4):3184-97
5) Sharp JA, et al.  (2002) Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae. Genes Dev 16(1):85-100
6) Krude T  (2002) Chromatin assembly: the kinetochore connection. Curr Biol 12(7):R256-8
7) Zabaronick SR and Tyler JK  (2005) The histone chaperone anti-silencing function 1 is a global regulator of transcription independent of passage through S phase. Mol Cell Biol 25(2):652-60
8) Zhou H, et al.  (2006) Chromatin assembly factor 1 interacts with histone H3 methylated at lysine 79 in the processes of epigenetic silencing and DNA repair. Biochemistry 45(9):2852-61
9) Kim JA and Haber JE  (2009) Chromatin assembly factors Asf1 and CAF-1 have overlapping roles in deactivating the DNA damage checkpoint when DNA repair is complete. Proc Natl Acad Sci U S A 106(4):1151-6
10) Kim HJ, et al.  (2009) Potential role of the histone chaperone, CAF-1, in transcription. BMB Rep 42(4):227-31
11) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30
12) Marquardt S, et al.  (2014) A chromatin-based mechanism for limiting divergent noncoding transcription. Cell 157(7):1712-23