BUR2/YLR226W Summary Help

Standard Name BUR2 1
Systematic Name YLR226W
Alias CST4
Feature Type ORF, Verified
Description Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise the CDK-cyclin BUR kinase complex which is involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II (Rpo21p); BUR kinase is also involved in the recruitment of Spt6p to the CTD at the onset of transcription (2, 3, 4, 5)
Name Description Bypass UAS Requirement 1
Chromosomal Location
ChrXII:589354 to 590541 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All BUR2 GO evidence and references
  View Computational GO annotations for BUR2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
null
overexpression
unspecified
Large-scale survey
null
overexpression
Resources
89 total interaction(s) for 57 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 16
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 9
  • PCA: 2
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 2
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 2
  • Synthetic Growth Defect: 15
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 33
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 395
Molecular Weight (Da) 45,720
Isoelectric Point (pI) 7.76
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:589354 to 590541 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1188 589354..590541 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004216
References cited on this page View Complete Literature Guide for BUR2
1) Prelich G and Winston F  (1993) Mutations that suppress the deletion of an upstream activating sequence in yeast: involvement of a protein kinase and histone H3 in repressing transcription in vivo. Genetics 135(3):665-76
2) Yao S, et al.  (2000) BUR1 and BUR2 encode a divergent cyclin-dependent kinase-cyclin complex important for transcription in vivo. Mol Cell Biol 20(19):7080-7
3) Yao S and Prelich G  (2002) Activation of the Bur1-Bur2 cyclin-dependent kinase complex by Cak1. Mol Cell Biol 22(19):6750-8
4) Pei Y, et al.  (2003) Interactions between fission yeast Cdk9, its cyclin partner Pch1, and mRNA capping enzyme Pct1 suggest an elongation checkpoint for mRNA quality control. J Biol Chem 278(9):7180-8
5) Dronamraju R and Strahl BD  (2014) A feed forward circuit comprising Spt6, Ctk1 and PAF regulates Pol II CTD phosphorylation and transcription elongation. Nucleic Acids Res 42(2):870-81