CDC45/YLR103C Summary Help

Standard Name CDC45 1
Systematic Name YLR103C
Alias SLD4 2
Feature Type ORF, Verified
Description DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; binds tightly to ssDNA, which disrupts interaction with the MCM helicase and stalls it during replication stress; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes (3, 4, 5)
Name Description Cell Division Cycle 1
Chromosomal Location
ChrXII:345941 to 343989 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All CDC45 GO evidence and references
  View Computational GO annotations for CDC45
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
conditional
null
reduction of function
repressible
Large-scale survey
conditional
null
reduction of function
repressible
Resources
254 total interaction(s) for 140 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 18
  • Affinity Capture-Western: 41
  • Co-fractionation: 10
  • Co-localization: 3
  • Co-purification: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 15
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Growth Defect: 5
  • Dosage Lethality: 1
  • Dosage Rescue: 5
  • Negative Genetic: 25
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 39
  • Synthetic Lethality: 76

Resources
Expression Summary
histogram
Resources
Length (a.a.) 650
Molecular Weight (Da) 74,248
Isoelectric Point (pI) 4.47
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:345941 to 343989 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1953 345941..343989 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004093
References cited on this page View Complete Literature Guide for CDC45
1) Moir D, et al.  (1982) Cold-sensitive cell-division-cycle mutants of yeast: isolation, properties, and pseudoreversion studies. Genetics 100(4):547-63
2) Kamimura Y, et al.  (1998) Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication. Mol Cell Biol 18(10):6102-9
3) Tye BK  (1999) MCM proteins in DNA replication. Annu Rev Biochem 68:649-86
4) McKie JM, et al.  (1998) Direct selection of conserved cDNAs from the DiGeorge critical region: isolation of a novel CDC45-like gene. Genome Res 8(8):834-41
5) Bruck I and Kaplan DL  (2013) Cdc45 protein-single-stranded DNA interaction is important for stalling the helicase during replication stress. J Biol Chem 288(11):7550-63