CBF1/YJR060W Summary Help

Standard Name CBF1 1
Systematic Name YJR060W
Alias CEP1 , CPF1
Feature Type ORF, Verified
Description Basic helix-loop-helix (bHLH) protein; forms homodimer to bind E-box consensus sequence CACGTG present at MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins, required for chromosome segregation; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5, 6, 7, 8, 9)
Name Description Centromere Binding Factor 1, 10
Gene Product Alias CP1 11 , 12 , 13
Chromosomal Location
ChrX:548759 to 549814 | ORF Map | GBrowse
Gbrowse
Genetic position: 40 cM
Gene Ontology Annotations All CBF1 GO evidence and references
  View Computational GO annotations for CBF1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulatory Role
Binding motifs Predicted CBF1 Binding Site Locations
    Regulatory modules predicted: stressResponse (487, 361)
    predicted: cellcycle (359, 311)
    predicted: stressResponse (487, 361)
    predicted: cellcycle (359, 311)
    Resources
    Mutant phenotypes All CBF1 Phenotype evidence and references
    Classical genetics
    null
    overexpression
    Large-scale survey
    null
    overexpression
    Resources
    interactions All CBF1 Interaction evidence and references
    303 total interaction(s) for 247 unique genes/features.
    Physical Interactions
    • Affinity Capture-MS: 6
    • Affinity Capture-RNA: 2
    • Affinity Capture-Western: 4
    • Biochemical Activity: 1
    • Co-localization: 3
    • Co-purification: 1
    • PCA: 5
    • Reconstituted Complex: 10
    • Two-hybrid: 4
    Genetic Interactions
    • Dosage Lethality: 1
    • Dosage Rescue: 2
    • Negative Genetic: 178
    • Phenotypic Enhancement: 1
    • Positive Genetic: 41
    • Synthetic Growth Defect: 16
    • Synthetic Lethality: 21
    • Synthetic Rescue: 7
    Resources
    Expression Summary
    histogram
    Resources
    Protein Information All CBF1 Protein evidence and references
    Localization
    Phosphorylation PhosphoGRID | PhosphoPep Database
    Structure
    Homologs
    sequence information
    ChrX:548759 to 549814 | ORF Map | GBrowse
    SGD ORF map
    Genetic position: 40 cM
    Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
    Subfeature details
    Relative
    Coordinates    		 Chromosomal
    Coordinates    		 Most Recent Updates
    Coordinates Sequence
    CDS 1..1056 548759..549814 2011-02-03 1996-07-31
    Retrieve sequences
    Analyze Sequence
    S288C only
    S288C vs. other species
    S288C vs. other strains
    Resources
    External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
    Primary SGDIDS000003821
    References cited on this page View Complete Literature Guide for CBF1
    1) Cai M and Davis RW  (1990) Yeast centromere binding protein CBF1, of the helix-loop-helix protein family, is required for chromosome stability and methionine prototrophy. Cell 61(3):437-46
    2) Kuras L, et al.  (1996) A heteromeric complex containing the centromere binding factor 1 and two basic leucine zipper factors, Met4 and Met28, mediates the transcription activation of yeast sulfur metabolism. EMBO J 15(10):2519-29
    3) Mellor J, et al.  (1991) DNA binding of CPF1 is required for optimal centromere function but not for maintaining methionine prototrophy in yeast. Nucleic Acids Res 19(11):2961-9
    4) Wieland G, et al.  (2001) Determination of the binding constants of the centromere protein Cbf1 to all 16 centromere DNAs of Saccharomyces cerevisiae. Nucleic Acids Res 29(5):1054-60
    5) Moreau JL, et al.  (2003) Regulated displacement of TBP from the PHO8 promoter in vivo requires Cbf1 and the Isw1 chromatin remodeling complex. Mol Cell 11(6):1609-20
    6) Kent NA, et al.  (2004) Cbf1p is required for chromatin remodeling at promoter-proximal CACGTG motifs in yeast. J Biol Chem 279(26):27116-23
    7) Zhou X and O'Shea EK  (2011) Integrated Approaches Reveal Determinants of Genome-wide Binding and Function of the Transcription Factor Pho4. Mol Cell 42(6):826-36
    8) McIsaac RS, et al.  (2012) Perturbation-based analysis and modeling of combinatorial regulation in the yeast sulfur assimilation pathway. Mol Biol Cell 23(15):2993-3007
    9) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
    10) Thomas D, et al.  (1992) MET4, a leucine zipper protein, and centromere-binding factor 1 are both required for transcriptional activation of sulfur metabolism in Saccharomyces cerevisiae. Mol Cell Biol 12(4):1719-27
    11) Baker RE and Masison DC  (1990) Isolation of the gene encoding the Saccharomyces cerevisiae centromere-binding protein CP1. Mol Cell Biol 10(6):2458-67
    12) Baker RE, et al.  (1989) Purification of the yeast centromere binding protein CP1 and a mutational analysis of its binding site. J Biol Chem 264(18):10843-50
    13) Bram RJ and Kornberg RD  (1987) Isolation of a Saccharomyces cerevisiae centromere DNA-binding protein, its human homolog, and its possible role as a transcription factor. Mol Cell Biol 7(1):403-9
    14) Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66
    15) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104
    16) Zhu J and Zhang MQ  (1999) SCPD: a promoter database of the yeast Saccharomyces cerevisiae. Bioinformatics 15(7-8):607-11
    17) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87