BUD32/YGR262C Summary Help

Standard Name BUD32 1
Systematic Name YGR262C
Alias LDB14
Feature Type ORF, Verified
Description Protein kinase; component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; Pyrococcus Bud32 ortholog functions as a P-loop ATPase rather than a protein kinase in the context of the complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription (1, 2, 3, 4, 5, 6, 7)
Name Description BUD site selection 1
Chromosomal Location
ChrVII:1017759 to 1016974 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All BUD32 GO evidence and references
  View Computational GO annotations for BUD32
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
reduction of function
Large-scale survey
158 total interaction(s) for 99 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 39
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 7
  • Biochemical Activity: 5
  • Far Western: 2
  • PCA: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Growth Defect: 34
  • Dosage Lethality: 1
  • Negative Genetic: 44
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 2
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 261
Molecular Weight (Da) 29,936
Isoelectric Point (pI) 9.15
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:1017759 to 1016974 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..786 1017759..1016974 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003494
References cited on this page View Complete Literature Guide for BUD32
1) Ni L and Snyder M  (2001) A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell 12(7):2147-70
2) Stocchetto S, et al.  (1997) Biochemical evidence that Saccharomyces cerevisiae YGR262c gene, required for normal growth, encodes a novel Ser/Thr-specific protein kinase. FEBS Lett 414(1):171-5
3) Downey M, et al.  (2006) A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator. Cell 124(6):1155-68
4) Kisseleva-Romanova E, et al.  (2006) Yeast homolog of a cancer-testis antigen defines a new transcription complex. EMBO J 25(15):3576-85
5) Srinivasan M, et al.  (2011) The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A. EMBO J 30(5):873-81
6) Hu Y, et al.  (2013) Telomerase-null survivor screening identifies novel telomere recombination regulators. PLoS Genet 9(1):e1003208
7) Perrochia L, et al.  (2013) Functional assignment of KEOPS/EKC complex subunits in the biosynthesis of the universal t6A tRNA modification. Nucleic Acids Res 41(20):9484-99