CYS4/YGR155W Summary Help

Standard Name CYS4 1
Systematic Name YGR155W
Alias NHS5 2 , STR4 3 , VMA41 4
Feature Type ORF, Verified
Description Cystathionine beta-synthase; catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria (1, 5, 6, 7, 8)
Name Description CYStathionine beta-synthase
Chromosomal Location
ChrVII:798543 to 800066 | ORF Map | GBrowse
Gbrowse
Genetic position: 87.14 cM
Gene Ontology Annotations All CYS4 GO evidence and references
  View Computational GO annotations for CYS4
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 19 genes
Resources
Pathways
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
153 total interaction(s) for 139 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 44
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 1
  • Co-crystal Structure: 1
  • PCA: 2
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 64
  • Positive Genetic: 29
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 507
Molecular Weight (Da) 56,021
Isoelectric Point (pI) 6.66
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:798543 to 800066 | ORF Map | GBrowse
SGD ORF map
Genetic position: 87.14 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1524 798543..800066 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003387
References cited on this page View Complete Literature Guide for CYS4
1) Ono B, et al.  (1988) Cysteine biosynthesis in Saccharomyces cerevisiae: mutation that confers cystathionine beta-synthase deficiency. J Bacteriol 170(12):5883-9
2) Skala J, et al.  (1995) The sequence of a 27 kb segment on the right arm of chromosome VII from Saccharomyces cerevisiae reveals MOL1, NAT2, RPL30B, RSR1, CYS4, PEM1/CHO2, NSR1 genes and ten new open reading frames. Yeast 11(14):1421-7
3) Cherest H, et al.  (1993) Cysteine biosynthesis in Saccharomyces cerevisiae occurs through the transsulfuration pathway which has been built up by enzyme recruitment. J Bacteriol 175(17):5366-74
4) Oluwatosin YE and Kane PM  (1997) Mutations in the CYS4 gene provide evidence for regulation of the yeast vacuolar H+-ATPase by oxidation and reduction in vivo. J Biol Chem 272(44):28149-57
5) Ono B, et al.  (1994) Purification and properties of Saccharomyces cerevisiae cystathionine beta-synthase. Yeast 10(3):333-9
6) Shan X and Kruger WD  (1998) Correction of disease-causing CBS mutations in yeast. Nat Genet 19(1):91-3
7) Singh S, et al.  (2009) Relative Contributions of Cystathionine {beta}-Synthase and {gamma}-Cystathionase to H2S Biogenesis via Alternative Trans-sulfuration Reactions. J Biol Chem 284(33):22457-66
8) Hoose SA, et al.  (2012) A systematic analysis of cell cycle regulators in yeast reveals that most factors act independently of cell size to control initiation of division. PLoS Genet 8(3):e1002590