FZF1/YGL254W Summary Help

Standard Name FZF1 1
Systematic Name YGL254W
Alias NRC299 , RSU1 2 , SUL1 3
Feature Type ORF, Verified
Description Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress (1, 4, 5, 6 and see Summary Paragraph)
Name Description Five Zinc Fingers 1
Chromosomal Location
ChrVII:22304 to 23203 | ORF Map | GBrowse
Gene Ontology Annotations All FZF1 GO evidence and references
  View Computational GO annotations for FZF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 2 genes
Regulators 4 genes
Classical genetics
Large-scale survey
29 total interaction(s) for 25 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 2
  • Protein-RNA: 1
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 8
  • Positive Genetic: 1
  • Synthetic Growth Defect: 2
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 299
Molecular Weight (Da) 33,994
Isoelectric Point (pI) 7.77
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:22304 to 23203 | ORF Map | GBrowse
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..900 22304..23203 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003223

FZF1 encodes a non-essential constitutively-expressed transcription factor involved in sulfite metabolism which contains five zinc fingers of the Cys2His2 class, three of which are positioned in tandem at the N-terminus (1, 4, 5). The fourth and fifth zinc fingers follow after interruptions of 61 and 66 amino acids, respectively (1). The sole Fzf1p target identified thus far is SSU1, which encodes a plasma membrane sulfite pump (5). Fzf1p binds directly to the SSU1 promoter, and its first zinc finger region is absolutely required for DNA binding; the fourth and fifth zinc fingers appear to be dispensable for DNA binding (5).

Multicopy FZF1 suppresses the sulfite sensitive phenotype of grr1 strains, but not the glucose derepression or aberrant cell morphology. Multicopy FZF1 was also found to suppress the sensitivity of a number of other unrelated sulfite-sensitive mutants, but not that of ssu1 or met1 (4). Some specific mutations in FZF1 conferring sulfite resistance have been identified. A C to G transversion at position 538 causing the substitution of an aspartic acid for a histidine in the fourth zinc finger directly affects its structure, thereby conferring sulfite resistance (3). Also, cysteine 57 in wild-type Fzf1p was found to be replaced by tyrosine in the dominant Fzf1-4p, which also confers sulfite resistance. Cells expressing FZF1-4 accumulate less intracellular sulfite due to hyperactivation of SSU1 (2, 7).

Last updated: 2005-02-25 Contact SGD

References cited on this page View Complete Literature Guide for FZF1
1) Breitwieser W, et al.  (1993) Identification of a gene encoding a novel zinc finger protein in Saccharomyces cerevisiae. Yeast 9(5):551-6
2) Park H, et al.  (1999) Use of sulfite resistance in Saccharomyces cerevisiae as a dominant selectable marker. Curr Genet 36(6):339-44
3) Casalone E, et al.  (1994) Cloning and characterization of a sulphite-resistance gene of Saccharomyces cerevisiae. Yeast 10(8):1101-10
4) Avram D and Bakalinsky AT  (1996) Multicopy FZF1 (SUL1) suppresses the sulfite sensitivity but not the glucose derepression or aberrant cell morphology of a grr1 mutant of Saccharomyces cerevisiae. Genetics 144(2):511-21
5) Avram D, et al.  (1999) Fzf1p of Saccharomyces cerevisiae is a positive regulator of SSU1 transcription and its first zinc finger region is required for DNA binding. Yeast 15(6):473-80
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7) Park H and Bakalinsky AT  (2000) SSU1 mediates sulphite efflux in Saccharomyces cerevisiae. Yeast 16(10):881-8
8) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87