DOT1/YDR440W Summary Help

Standard Name DOT1 1
Systematic Name YDR440W
Alias PCH1 2
Feature Type ORF, Verified
Description Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response (2, 3, 4, 5)
Name Description Disruptor Of Telomeric silencing 1
Gene Product Alias KMT4 6
Chromosomal Location
ChrIV:1342493 to 1344241 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All DOT1 GO evidence and references
  View Computational GO annotations for DOT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 1958 genes
Regulators 59 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
252 total interaction(s) for 162 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 6
  • Biochemical Activity: 14
  • Co-localization: 3
  • Far Western: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 4
  • Dosage Rescue: 2
  • Negative Genetic: 127
  • Phenotypic Enhancement: 18
  • Phenotypic Suppression: 7
  • Positive Genetic: 2
  • Synthetic Growth Defect: 34
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 8
  • Synthetic Rescue: 16

Resources
Expression Summary
histogram
Resources
Length (a.a.) 582
Molecular Weight (Da) 66,201
Isoelectric Point (pI) 9.76
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1342493 to 1344241 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1749 1342493..1344241 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002848
References cited on this page View Complete Literature Guide for DOT1
1) Singer MS, et al.  (1998) Identification of high-copy disruptors of telomeric silencing in Saccharomyces cerevisiae. Genetics 150(2):613-32
2) San-Segundo PA and Roeder GS  (2000) Role for the silencing protein Dot1 in meiotic checkpoint control. Mol Biol Cell 11(10):3601-15
3) van Leeuwen F, et al.  (2002) Dot1p modulates silencing in yeast by methylation of the nucleosome core. Cell 109(6):745-56
4) Game JC, et al.  (2005) X-ray survival characteristics and genetic analysis for nine Saccharomyces deletion mutants that show altered radiation sensitivity. Genetics 169(1):51-63
5) Wysocki R, et al.  (2005) Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9. Mol Cell Biol 25(19):8430-43
6) Allis CD, et al.  (2007) New nomenclature for chromatin-modifying enzymes. Cell 131(4):633-6