DOT1/YDR440W Summary Help

Standard Name DOT1 1
Systematic Name YDR440W
Alias PCH1 2
Feature Type ORF, Verified
Description Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response (2, 3, 4, 5)
Name Description Disruptor Of Telomeric silencing 1
Gene Product Alias KMT4 6
Chromosomal Location
ChrIV:1342493 to 1344241 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All DOT1 GO evidence and references
  View Computational GO annotations for DOT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
257 total interaction(s) for 163 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 6
  • Biochemical Activity: 14
  • Co-localization: 3
  • Far Western: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 4
  • Dosage Rescue: 2
  • Negative Genetic: 127
  • Phenotypic Enhancement: 18
  • Phenotypic Suppression: 8
  • Positive Genetic: 4
  • Synthetic Growth Defect: 35
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 8
  • Synthetic Rescue: 16

Resources
Expression Summary
histogram
Resources
Length (a.a.) 582
Molecular Weight (Da) 66,201
Isoelectric Point (pI) 9.76
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1342493 to 1344241 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1749 1342493..1344241 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002848
References cited on this page View Complete Literature Guide for DOT1
1) Singer MS, et al.  (1998) Identification of high-copy disruptors of telomeric silencing in Saccharomyces cerevisiae. Genetics 150(2):613-32
2) San-Segundo PA and Roeder GS  (2000) Role for the silencing protein Dot1 in meiotic checkpoint control. Mol Biol Cell 11(10):3601-15
3) van Leeuwen F, et al.  (2002) Dot1p modulates silencing in yeast by methylation of the nucleosome core. Cell 109(6):745-56
4) Game JC, et al.  (2005) X-ray survival characteristics and genetic analysis for nine Saccharomyces deletion mutants that show altered radiation sensitivity. Genetics 169(1):51-63
5) Wysocki R, et al.  (2005) Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9. Mol Cell Biol 25(19):8430-43
6) Allis CD, et al.  (2007) New nomenclature for chromatin-modifying enzymes. Cell 131(4):633-6