Standard Name	EAP1
Systematic Name	YKL204W
Feature Type	ORF, Verified
Description	eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade (1, 2, 3, 4)
Name Description	EIF4E-Associated Protein 1, 2

Chromosomal Location	ChrXI:53704 to 55602 | ORF Map | GBrowse

	View Computational GO annotations for EAP1
Molecular Function
Manually curated	eukaryotic initiation factor 4E binding (IPI)
Biological Process
Manually curated	negative regulation of translation (IDA, IMP)
Cellular Component
Manually curated	cytoplasmic stress granule (IDA) mRNA cap binding complex (IPI)

Classical genetics
null	utilization of nitrogen source: decreased vegetative growth: decreased rate
Large-scale survey
null	anaerobic growth: decreased cell size: increased competitive fitness: decreased endocytosis: decreased fermentative growth: decreased rate haploinsufficient heat sensitivity: increased ionic stress resistance: decreased metal resistance: decreased resistance to hydroxyurea: decreased resistance to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO): decreased resistance to sirolimus: decreased resistance to wortmannin: increased resistance to cycloheximide: decreased resistance to amitrole: decreased resistance to cisplatin: decreased resistance to benomyl: decreased resistance to hydrogen chloride: decreased resistance to sorbate: decreased resistance to dimethyl sulfoxide: decreased telomere length: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	75 total interaction(s) for 63 unique genes/features.
Physical Interactions	Affinity Capture-MS: 14 Affinity Capture-RNA: 2 Affinity Capture-Western: 10 Co-fractionation: 1 PCA: 1 Two-hybrid: 2
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 1 Negative Genetic: 12 Phenotypic Enhancement: 1 Positive Genetic: 2 Synthetic Growth Defect: 10 Synthetic Lethality: 13 Synthetic Rescue: 5
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXI:53704 to 55602 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1899 53704..55602 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001687