CSE4/YKL049C Summary Help

Standard Name CSE4 1
Systematic Name YKL049C
Alias CSL2 2
Feature Type ORF, Verified
Description Centromere protein that resembles histone H3; associated with promoters, accessible chromatin and RNA polymerase II-bound regions; phosphorylated Cse4p associates with centromeres; required for proper kinetochore function; levels regulated by E3 ubiquitin ligase Psh1p; phosphorylation of Cse4p may destabilize defective kinetochores to promote bi-orientation; ubiquitination of N terminus regulates Cse4p proteolysis for faithful chromosome segregation; human CENP-A homolog (1, 3, 4, 5, 6, 7, 8)
Name Description Chromosome SEgregation 1
Chromosomal Location
ChrXI:346764 to 346075 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All CSE4 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
457 total interaction(s) for 352 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 314
  • Affinity Capture-Western: 36
  • Biochemical Activity: 4
  • Co-crystal Structure: 1
  • Co-localization: 10
  • FRET: 1
  • PCA: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Lethality: 10
  • Dosage Rescue: 12
  • Negative Genetic: 2
  • Phenotypic Suppression: 4
  • Synthetic Growth Defect: 29
  • Synthetic Lethality: 16
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 229
Molecular Weight (Da) 26,841
Isoelectric Point (pI) 10.13
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:346764 to 346075 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 2003-01-10
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..690 346764..346075 2011-02-03 2003-01-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001532
References cited on this page View Complete Literature Guide for CSE4
1) Stoler S, et al.  (1995) A mutation in CSE4, an essential gene encoding a novel chromatin-associated protein in yeast, causes chromosome nondisjunction and cell cycle arrest at mitosis. Genes Dev 9(5):573-86
2) Baker RE, et al.  (1998) Mutations synthetically lethal with cep1 target S. cerevisiae kinetochore components. Genetics 149(1):73-85
3) Meluh PB, et al.  (1998) Cse4p is a component of the core centromere of Saccharomyces cerevisiae. Cell 94(5):607-13
4) Ranjitkar P, et al.  (2010) An E3 Ubiquitin Ligase Prevents Ectopic Localization of the Centromeric Histone H3 Variant via the Centromere Targeting Domain. Mol Cell 40(3):455-64
5) Hewawasam G, et al.  (2010) Psh1 Is an E3 Ubiquitin Ligase that Targets the Centromeric Histone Variant Cse4. Mol Cell 40(3):444-54
6) Lefrancois P, et al.  (2013) Centromere-like regions in the budding yeast genome. PLoS Genet 9(1):e1003209
7) Au WC, et al.  (2013) A novel role of the N terminus of budding yeast histone h3 variant cse4 in ubiquitin-mediated proteolysis. Genetics 194(2):513-8
8) Boeckmann L, et al.  (2013) Phosphorylation of centromeric histone H3 variant regulates chromosome segregation in Saccharomyces cerevisiae. Mol Biol Cell 24(12):2034-2044