DSN1/YIR010W Summary Help

Standard Name DSN1 1
Systematic Name YIR010W
Feature Type ORF, Verified
Description Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; phosphorylation of Dsn1p promotes interaction between outer and inner kinetochore proteins; N-terminal end interacts with monopolin subunit Csm1p and is essential for meiotic but not mitotic chromosome segregation; important for chromosome segregation; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND); modified by sumoylation (1, 2, 3, 4, 5)
Name Description Dosage Suppressor of NNF1 1
Chromosomal Location
ChrIX:375431 to 377161 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All DSN1 GO evidence and references
  View Computational GO annotations for DSN1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
conditional
reduction of function
Large-scale survey
conditional
null
reduction of function
repressible
Resources
683 total interaction(s) for 543 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 521
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 32
  • Biochemical Activity: 1
  • Co-crystal Structure: 30
  • Co-fractionation: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Rescue: 27
  • Negative Genetic: 43
  • Positive Genetic: 5
  • Synthetic Lethality: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 576
Molecular Weight (Da) 65,692
Isoelectric Point (pI) 5.11
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:375431 to 377161 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1731 375431..377161 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001449
References cited on this page View Complete Literature Guide for DSN1
1) Euskirchen GM  (2002) Nnf1p, Dsn1p, Mtw1p, and Nsl1p: a new group of proteins important for chromosome segregation in Saccharomyces cerevisiae. Eukaryot Cell 1(2):229-40
2) De Wulf P, et al.  (2003) Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes. Genes Dev 17(23):2902-21
3) Yong-Gonzales V, et al.  (2012) The Smc5-Smc6 Complex Regulates Recombination at Centromeric Regions and Affects Kinetochore Protein Sumoylation during Normal Growth. PLoS One 7(12):e51540
4) Akiyoshi B, et al.  (2013) The aurora B kinase promotes inner and outer kinetochore interactions in budding yeast. Genetics 194(3):785-9
5) Sarkar S, et al.  (2013) Monopolin Subunit Csm1 Associates with MIND Complex to Establish Monopolar Attachment of Sister Kinetochores at Meiosis I. PLoS Genet 9(7):e1003610