LYS12/YIL094C Summary

Standard Name	LYS12 1
Systematic Name	YIL094C
Alias	LYS10 , LYS11
Feature Type	ORF, Verified
Description	Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate (2 and see Summary Paragraph)
Name Description	LYSine requiring 1

Chromosomal Location	ChrIX:187632 to 186517 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All LYS12 GO evidence and references

	View Computational GO annotations for LYS12
Molecular Function
Manually curated	homoisocitrate dehydrogenase activity (IDA)
Biological Process
Manually curated	lysine biosynthetic process (IC)
Cellular Component
High-throughput	mitochondrion (IDA)

Pathways	lysine biosynthesis

Mutant phenotypes All LYS12 Phenotype evidence and references

Classical genetics
null	glycogen accumulation: increased chronological lifespan: increased resistance to sirolimus: increased
Large-scale survey
null	competitive fitness: decreased haploproficient replicative lifespan: increased vacuolar morphology: abnormal
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All LYS12 Interaction evidence and references

	74 total interaction(s) for 68 unique genes/features.
Physical Interactions	Affinity Capture-MS: 58 Affinity Capture-RNA: 2
Genetic Interactions	Synthetic Growth Defect: 2 Synthetic Lethality: 11 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All LYS12 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIX:187632 to 186517 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1994-12-10

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1116	187632..186517	2011-02-03	1994-12-10

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001356

SUMMARY PARAGRAPH for LYS12

About lysine biosynthesis

S. cerevisiae synthesizes the essential amino acid L-lysine via the L-alpha-aminoadipic acid pathway instead of the diaminopmelate pathway (3). Originally proposed to be characteristic of fungi, recent studies suggest prokaryotes also synthesize lysine via the alpha-aminoadipic acid pathway (4). Intermediates in this pathway are often incorporated into secondary metabolites. For example, it has been well- studied that alpha-aminoadipate is required for penicillin production (3). Regulation of the lysine biosynthetic pathway in S. cerevisiae is an interaction between general amino acid control (via Gcn4p) (5), feedback inhibition of homocitrate synthase activity by lysine (6), and induction of Lys14p by alpha-aminoadipate semialdehyde (7).

Last updated: 2007-10-04

References cited on this page View Complete Literature Guide for LYS12

1)	Jonkers, H., et al. (1998) Identification of YIL094C as the gene for homo-isocitrate dehydrogenase (LYS12) in Saccharomyces cerevisiae
2)	Strassman M and Ceci LN (1965) Enzymatic formation of alpha-ketoadipic acid from homoisocitric acid. J Biol Chem 240(11):4357-61
3)	Zabriskie TM and Jackson MD (2000) Lysine biosynthesis and metabolism in fungi. Nat Prod Rep 17(1):85-97
4)	Nishida H and Nishiyama M (2000) What is characteristic of fungal lysine synthesis through the alpha-aminoadipate pathway? J Mol Evol 51(3):299-302
5)	Hinnebusch A (1992) "General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae". Pp. 319-414 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
6)	Feller A, et al. (1999) In Saccharomyces cerevisae, feedback inhibition of homocitrate synthase isoenzymes by lysine modulates the activation of LYS gene expression by Lys14p. Eur J Biochem 261(1):163-70
7)	El Alami M, et al. (2000) Characterisation of a tripartite nuclear localisation sequence in the regulatory protein Lys14 of Saccharomyces cerevisiae. Curr Genet 38(2):78-86