SRB2/YHR041C Summary Help

Standard Name SRB2 1
Systematic Name YHR041C
Alias HRS2 , MED20 2
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance (1, 3, 4, 5, 6, 7)
Name Description Suppressor of RNA polymerase B 1
Chromosomal Location
ChrVIII:189864 to 189131 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All SRB2 GO evidence and references
  View Computational GO annotations for SRB2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 48 genes
Regulators 7 genes
Classical genetics
Large-scale survey
517 total interaction(s) for 303 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 67
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 22
  • Co-crystal Structure: 2
  • Co-purification: 24
  • Reconstituted Complex: 6

Genetic Interactions
  • Negative Genetic: 274
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 5
  • Positive Genetic: 47
  • Synthetic Growth Defect: 33
  • Synthetic Lethality: 16
  • Synthetic Rescue: 11

Expression Summary
Length (a.a.) 210
Molecular Weight (Da) 22,894
Isoelectric Point (pI) 5.15
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVIII:189864 to 189131 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..14 189864..189851 2011-02-03 1996-07-31
Intron 15..115 189850..189750 2011-02-03 1996-07-31
CDS 116..734 189749..189131 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001083
References cited on this page View Complete Literature Guide for SRB2
1) Nonet ML and Young RA  (1989) Intragenic and extragenic suppressors of mutations in the heptapeptide repeat domain of Saccharomyces cerevisiae RNA polymerase II. Genetics 123(4):715-24
2) Bourbon HM, et al.  (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
3) Thompson CM, et al.  (1993) A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast. Cell 73(7):1361-75
4) Myers LC, et al.  (1998) The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev 12(1):45-54
5) Kim YJ, et al.  (1994) A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II. Cell 77(4):599-608
6) Kornberg RD  (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9
7) Askree SH, et al.  (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63