KAP123/YER110C Summary

KAP123/YER110C Summary

Standard Name	KAP123
Systematic Name	YER110C
Alias	YRB4
Feature Type	ORF, Verified
Description	Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 (1, 2, 3)
Name Description	KAryoPherin

Chromosomal Location	ChrV:382103 to 378762 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All KAP123 GO evidence and references

	View Computational GO annotations for KAP123
Molecular Function
Manually curated	nuclear localization sequence binding (IDA) protein transporter activity (IMP, IPI)
Biological Process
Manually curated	NLS-bearing substrate import into nucleus (IMP) protein import into nucleus (IMP) regulation of pseudohyphal growth (IMP) ribosomal protein import into nucleus (IMP)
Cellular Component
Manually curated	cytoplasm (IDA) nuclear pore (IDA) nucleus (IDA)
High-throughput	integral to membrane (ISM)

Mutant phenotypes All KAP123 Phenotype evidence and references

Classical genetics
null	chromosome segregation: abnormal nuclear import: normal nuclear import: decreased pseudohyphal growth: decreased resistance to benomyl: decreased resistance to sirolimus: decreased
repressible	nuclear import: decreased
Large-scale survey
null	bud morphology: abnormal competitive fitness: decreased dessication resistance: decreased filamentous growth: decreased heat sensitivity: increased invasive growth: absent resistance to benomyl: decreased resistance to cycloheximide: decreased resistance to glycolaldehyde: decreased resistance to sodium arsenite: decreased resistance to arsenite(3-): decreased resistance to ethanol: decreased respiratory growth: decreased rate stress resistance: increased transposable element transposition: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All KAP123 Interaction evidence and references

	262 total interaction(s) for 177 unique genes/features.
Physical Interactions	Affinity Capture-MS: 144 Affinity Capture-RNA: 2 Affinity Capture-Western: 19 Co-fractionation: 1 Co-localization: 1 Co-purification: 2 Protein-peptide: 2 Reconstituted Complex: 25 Two-hybrid: 6
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 39 Phenotypic Enhancement: 1 Positive Genetic: 12 Synthetic Growth Defect: 5 Synthetic Lethality: 1 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Mass Spec

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All KAP123 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrV:382103 to 378762 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3342	382103..378762	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000912

References cited on this page View Complete Literature Guide for KAP123

1)	Schlenstedt G, et al. (1997) Yrb4p, a yeast ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus. EMBO J 16(20):6237-49
2)	Sydorskyy Y, et al. (2003) Intersection of the Kap123p-mediated nuclear import and ribosome export pathways. Mol Cell Biol 23(6):2042-54
3)	Mosammaparast N, et al. (2002) Pathways mediating the nuclear import of histones H3 and H4 in yeast. J Biol Chem 277(1):862-8