CIN8/YEL061C Summary

CIN8/YEL061C Summary

Standard Name	CIN8
Systematic Name	YEL061C
Alias	KSL2 , SDS15 1
Feature Type	ORF, Verified
Description	Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation (2, 3, 4)
Name Description	Chromosome INstability 5

Chromosomal Location	ChrV:39537 to 36535 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All CIN8 GO evidence and references

	View Computational GO annotations for CIN8
Molecular Function
Manually curated	minus-end-directed microtubule motor activity (IDA) plus-end-directed microtubule motor activity (IDA)
Biological Process
Manually curated	microtubule depolymerization (IMP) mitotic anaphase B (IMP) mitotic sister chromatid segregation (IGI) mitotic spindle organization in nucleus (TAS) spindle pole body separation (IGI, IMP)
Cellular Component
Manually curated	astral microtubule (IMP) condensed nuclear chromosome kinetochore (IDA) kinesin complex (TAS) kinetochore microtubule (IDA) spindle microtubule (IDA)
High-throughput	mitochondrion (IDA)

Mutant phenotypes All CIN8 Phenotype evidence and references

Classical genetics
null	cell cycle progression in anaphase: delayed chromosome segregation: abnormal chromosome/plasmid maintenance: abnormal colony sectoring: increased resistance to cincreasin: decreased spindle morphology: abnormal
overexpression	cell cycle passage through the metaphase-anaphase transition: delayed colony sectoring: increased resistance to benomyl: decreased spindle morphology: abnormal
Large-scale survey
null	cell size: increased competitive fitness: decreased dessication resistance: decreased fermentative growth: decreased rate haploinsufficient heat sensitivity: increased resistance to dimethyl sulfoxide: decreased resistance to sodium arsenite: decreased resistance to wortmannin: normal resistance to sirolimus: decreased resistance to benomyl: decreased resistance to St. John's Wort extract: decreased stress resistance: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
overexpression	colony sectoring: increased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All CIN8 Interaction evidence and references

	515 total interaction(s) for 250 unique genes/features.
Physical Interactions	Affinity Capture-MS: 10 Affinity Capture-Western: 3 Biochemical Activity: 1 PCA: 1 Reconstituted Complex: 2 Two-hybrid: 12
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 4 Negative Genetic: 172 Phenotypic Enhancement: 10 Phenotypic Suppression: 10 Positive Genetic: 6 Synthetic Growth Defect: 68 Synthetic Lethality: 203 Synthetic Rescue: 12
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All CIN8 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrV:39537 to 36535 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2003-09-22 | Sequence: 2003-09-22

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3003	39537..36535	2003-09-22	2003-09-22

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000787

References cited on this page View Complete Literature Guide for CIN8

1)	Sussel L, et al. (1995) Suppressors of defective silencing in yeast: effects on transcriptional repression at the HMR locus, cell growth and telomere structure. Genetics 141(3):873-88
2)	Hoyt MA, et al. (1992) Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly. J Cell Biol 118(1):109-20
3)	Saunders WS and Hoyt MA (1992) Kinesin-related proteins required for structural integrity of the mitotic spindle. Cell 70(3):451-8
4)	Roof DM, et al. (1992) Kinesin-related proteins required for assembly of the mitotic spindle. J Cell Biol 118(1):95-108
5)	Stearns T, et al. (1990) Yeast mutants sensitive to antimicrotubule drugs define three genes that affect microtubule function. Genetics 124(2):251-62