PRB1/YEL060C Summary Help

Standard Name PRB1
Systematic Name YEL060C
Alias CVT1
Feature Type ORF, Verified
Description Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress; PRB1 has a paralog, YSP3, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6)
Name Description PRoteinase B 7
Chromosomal Location
ChrV:41953 to 40046 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -48 cM
Gene Ontology Annotations All PRB1 GO evidence and references
  View Computational GO annotations for PRB1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All PRB1 Phenotype evidence and references
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Resources
interactions All PRB1 Interaction evidence and references
74 total interaction(s) for 73 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 22
  • Affinity Capture-RNA: 2
  • Two-hybrid: 2
Genetic Interactions
  • Negative Genetic: 38
  • Phenotypic Enhancement: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 3
  • Synthetic Rescue: 2
Resources
Expression Summary
histogram
Resources
Protein Information All PRB1 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:41953 to 40046 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: -48 cM
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1908 41953..40046 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000786
References cited on this page View Complete Literature Guide for PRB1
1) Moehle CM, et al.  (1987) Protease B of the lysosomelike vacuole of the yeast Saccharomyces cerevisiae is homologous to the subtilisin family of serine proteases. Mol Cell Biol 7(12):4390-9
2) Schu P, et al.  (1991) The proteinase yscB inhibitor (PB12) gene of yeast and studies on the function of its protein product. Eur J Biochem 197(1):1-7
3) Zubenko GS and Jones EW  (1981) Protein degradation, meiosis and sporulation in proteinase-deficient mutants of Saccharomyces cerevisiae. Genetics 97(1):45-64
4) Teichert U, et al.  (1989) Lysosomal (vacuolar) proteinases of yeast are essential catalysts for protein degradation, differentiation, and cell survival. J Biol Chem 264(27):16037-45
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Bauer R, et al.  (1976) Low and high pH form of cadmium carbonic anhydrase determined by nuclear quadrupole interaction. Biochemistry 15(2):334-42
7) Zubenko GS and Jones EW  (1979) Catabolite inactivation of gluconeogenic enzymes in mutants of yeast deficient in proteinase B. Proc Natl Acad Sci U S A 76(9):4581-5