Standard Name	AME1 1
Systematic Name	YBR211C
Alias	ARP100
Feature Type	ORF, Verified
Description	Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress (1, 2, 3, 4)
Name Description	Associated with Microtubules and Essential 1

Chromosomal Location	ChrII:647132 to 646158 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for AME1
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	attachment of spindle microtubules to kinetochore (IDA, IPI) protein localization to kinetochore (IMP)
Cellular Component
Manually curated	COMA complex (IDA) kinetochore (IDA) spindle pole body (NAS)
High-throughput	cytoplasm (IDA) nucleus (IDA) spindle pole body (IDA)

Classical genetics
conditional	cell cycle progression in anaphase: absent heat sensitivity: increased
null	inviable
Large-scale survey
conditional	colony sectoring: increased
null	inviable
overexpression	cell cycle progression: abnormal
repressible	cell cycle progression in G2 phase: abnormal chromosome/plasmid maintenance: decreased
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	190 total interaction(s) for 101 unique genes/features.
Physical Interactions	Affinity Capture-MS: 54 Affinity Capture-RNA: 1 Affinity Capture-Western: 12 Biochemical Activity: 2 Co-crystal Structure: 1 Co-fractionation: 1 PCA: 4 Reconstituted Complex: 9 Two-hybrid: 35
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 2 Negative Genetic: 47 Positive Genetic: 16 Synthetic Growth Defect: 1 Synthetic Lethality: 4
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Mass Spec | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrII:647132 to 646158 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..975 647132..646158 2011-02-03 1997-01-28
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000415