RHO3/YIL118W Summary Help

Standard Name RHO3 1
Systematic Name YIL118W
Feature Type ORF, Verified
Description Non-essential small GTPase of the Rho/Rac family of Ras-like proteins; involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p (2, 3 and see Summary Paragraph)
Name Description Ras HOmolog 1
Chromosomal Location
ChrIX:139752 to 140447 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RHO3 GO evidence and references
  View Computational GO annotations for RHO3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 15 genes
Resources
Classical genetics
conditional
null
Large-scale survey
conditional
null
reduction of function
Resources
116 total interaction(s) for 63 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 4
  • Biochemical Activity: 1
  • Protein-RNA: 1
  • Reconstituted Complex: 7
  • Two-hybrid: 10

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 38
  • Negative Genetic: 8
  • Phenotypic Enhancement: 1
  • Positive Genetic: 3
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 16
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 231
Molecular Weight (Da) 25,312
Isoelectric Point (pI) 4.73
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:139752 to 140447 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..696 139752..140447 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001380
SUMMARY PARAGRAPH for RHO3

RHO3 encodes a non-essential small GTPase in the Rho/Rac subfamily of Ras-like GTPases (4). Other S. cerevisiae Rho GTPases are encoded by RHO1, RHO2, RHO4, RHO5, and CDC42 (3). Like other eukaryotic Rho-type GTPases, Rho3p is involved in the establishment of cell polarity (3). Temperature sensitive rho3 mutants lose cell polarity at the restrictive temperature; a dominant RHO3 allele causes cold sensitivity and aberrant cell morphology (5). RHO3 interacts genetically with SEC4, which encodes a Rab-type small GTPase, suggesting that Rho3p may regulate polarized secretion (5, 3). rho3 and rho4 null mutations are synthetically lethal (4, 3). The GTPase activity of Rho3p is positively regulated by the GTPase activating protein (GAP) Rgd1p (2).

Last updated: 1999-11-24 Contact SGD

References cited on this page View Complete Literature Guide for RHO3
1) Matsui Y and Toh-e A  (1992) Isolation and characterization of two novel ras superfamily genes in Saccharomyces cerevisiae. Gene 114(1):43-9
2) Doignon F, et al.  (1999) The yeast Rgd1p is a GTPase activating protein of the Rho3 and rho4 proteins. FEBS Lett 459(3):458-62
3) Madden K and Snyder M  (1998) Cell polarity and morphogenesis in budding yeast. Annu Rev Microbiol 52():687-744
4) Matsui Y and Toh-E A  (1992) Yeast RHO3 and RHO4 ras superfamily genes are necessary for bud growth, and their defect is suppressed by a high dose of bud formation genes CDC42 and BEM1. Mol Cell Biol 12(12):5690-9
5) Imai J, et al.  (1996) Genetic analysis of the Saccharomyces cerevisiae RHO3 gene, encoding a rho-type small GTPase, provides evidence for a role in bud formation. Genetics 142(2):359-69