RHO1/YPR165W Summary Help

Standard Name RHO1 1
Systematic Name YPR165W
Feature Type ORF, Verified
Description GTP-binding protein of the rho subfamily of Ras-like proteins; involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) (2, 3, 4 and see Summary Paragraph)
Name Description Ras HOmolog 1
Chromosomal Location
ChrXVI:875368 to 875997 | ORF Map | GBrowse
Gene Ontology Annotations All RHO1 GO evidence and references
  View Computational GO annotations for RHO1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
149 total interaction(s) for 80 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 16
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • Co-fractionation: 4
  • Co-purification: 1
  • FRET: 1
  • PCA: 2
  • Reconstituted Complex: 11
  • Two-hybrid: 25

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 8
  • Dosage Rescue: 40
  • Negative Genetic: 1
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 5
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 2
  • Synthetic Rescue: 7

Expression Summary
Length (a.a.) 209
Molecular Weight (Da) 23,152
Isoelectric Point (pI) 6.07
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:875368 to 875997 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..630 875368..875997 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006369

RHO1 encodes an essential small GTPase in the Rho/Rac subfamily of Ras-like GTPases (5). Other S. cerevisiae Rho GTPases are encoded by RHO2, RHO3, RHO4, RHO5, and CDC42 (6). Like other eukaryotic Rho-type GTPases, Rho1p is involved in the establishment of cell polarity, playing a role in the reorganization of the actin cytoskeleton (6). Rho1p regulates protein kinase C (encoded by PKC1) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (encoded by FKS1 and GSC2) (3, 4). Rho1p is localized to the plasma membrane at sites of growth such as incipient bud sites, bud tips, and the bud neck during cytokinesis (5). The localization of Rho1p is aberrent in sla1 null mutant cells (7). At the restrictive temperature, some temperature-sensitive rho1 mutants arrest with small buds (5); other temperature-sensitive rho1 alleles cause arrest as unbudded cells (2). The GTPase activity of Rho1p is regulated by the GTPase activating proteins (GAPs) Bem2p, Sac7p, Bag2p, and Rdi1p, and the guanine nucleotide exchange factors (GEFs) Rom1p and Rom2p (6).

Last updated: 1999-11-24 Contact SGD

References cited on this page View Complete Literature Guide for RHO1
1) Madaule P, et al.  (1987) Characterization of two members of the rho gene family from the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 84(3):779-83
2) Drgonova J, et al.  (1999) The GTP-binding protein Rho1p is required for cell cycle progression and polarization of the yeast cell. J Cell Biol 146(2):373-87
3) Drgonova J, et al.  (1996) Rho1p, a yeast protein at the interface between cell polarization and morphogenesis. Science 272(5259):277-9
4) Qadota H, et al.  (1996) Identification of yeast Rho1p GTPase as a regulatory subunit of 1,3-beta-glucan synthase. Science 272(5259):279-81
5) Yamochi W, et al.  (1994) Growth site localization of Rho1 small GTP-binding protein and its involvement in bud formation in Saccharomyces cerevisiae. J Cell Biol 125(5):1077-93
6) Madden K and Snyder M  (1998) Cell polarity and morphogenesis in budding yeast. Annu Rev Microbiol 52():687-744
7) Ayscough KR, et al.  (1999) Sla1p is a functionally modular component of the yeast cortical actin cytoskeleton required for correct localization of both Rho1p-GTPase and Sla2p, a protein with talin homology. Mol Biol Cell 10(4):1061-75