RVS167/YDR388W Summary Help

Standard Name RVS167 1, 2
Systematic Name YDR388W
Feature Type ORF, Verified
Description Actin-associated protein with roles in endocytosis and exocytosis; interacts with Rvs161p to regulate actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; recruited to bud tips by Gyl1p and Gyp5p during polarized growth; homolog of mammalian amphiphysin (1, 3, 4, 5, 6)
Name Description Reduced Viability on Starvation 1
Chromosomal Location
ChrIV:1250186 to 1251634 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RVS167 GO evidence and references
  View Computational GO annotations for RVS167
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 11 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
871 total interaction(s) for 545 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 130
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 17
  • Biochemical Activity: 7
  • Co-localization: 3
  • Far Western: 2
  • FRET: 1
  • PCA: 8
  • Protein-peptide: 11
  • Reconstituted Complex: 8
  • Two-hybrid: 140

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Lethality: 2
  • Dosage Rescue: 2
  • Negative Genetic: 284
  • Phenotypic Enhancement: 18
  • Phenotypic Suppression: 5
  • Positive Genetic: 61
  • Synthetic Growth Defect: 75
  • Synthetic Lethality: 90
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 482
Molecular Weight (Da) 52,774
Isoelectric Point (pI) 5.89
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1250186 to 1251634 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1449 1250186..1251634 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002796
References cited on this page View Complete Literature Guide for RVS167
1) Bauer F, et al.  (1993) Alteration of a yeast SH3 protein leads to conditional viability with defects in cytoskeletal and budding patterns. Mol Cell Biol 13(8):5070-84
2) Crouzet, M., et al.  (1992) ; Personal Communication, Mortimer Map Edition 11
3) Balguerie A, et al.  (1999) Rvs167p, the budding yeast homolog of amphiphysin, colocalizes with actin patches. J Cell Sci 112 ( Pt 15)():2529-37
4) Lila T and Drubin DG  (1997) Evidence for physical and functional interactions among two Saccharomyces cerevisiae SH3 domain proteins, an adenylyl cyclase-associated protein and the actin cytoskeleton. Mol Biol Cell 8(2):367-85
5) Lombardi R and Riezman H  (2001) Rvs161p and Rvs167p, the two yeast amphiphysin homologs, function together in vivo. J Biol Chem 276(8):6016-22
6) Prigent M, et al.  (2011) The RabGAP proteins Gyp5p and Gyl1p recruit the BAR domain protein Rvs167p for polarized exocytosis. Traffic 12(8):1084-97