RUP1/YOR138C Summary Help

Standard Name RUP1 1
Systematic Name YOR138C
Feature Type ORF, Verified
Description Protein that regulates ubiquitination of Rsp5p; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress (1, 2, 3, 4)
Name Description Rsp5-Ubp2 interacting Protein 2
Chromosomal Location
ChrXV:586324 to 584309 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RUP1 GO evidence and references
  View Computational GO annotations for RUP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 13 genes
Resources
Large-scale survey
null
overexpression
Resources
56 total interaction(s) for 30 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 8
  • Co-purification: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Rescue: 1
  • Negative Genetic: 20
  • Positive Genetic: 2
  • Synthetic Growth Defect: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 671
Molecular Weight (Da) 75,360
Isoelectric Point (pI) 4.45
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:586324 to 584309 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2016 586324..584309 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005664
References cited on this page View Complete Literature Guide for RUP1
1) Kee Y, et al.  (2005) The Rsp5 ubiquitin ligase is coupled to and antagonized by the Ubp2 deubiquitinating enzyme. EMBO J 24(13):2414-24
2) Lam MH, et al.  (2009) Interaction of the Deubiquitinating Enzyme Ubp2 and the E3 Ligase Rsp5 Is Required for Transporter/Receptor Sorting in the Multivesicular Body Pathway. PLoS One 4(1):e4259
3) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
4) Lam MH and Emili A  (2013) Ubp2 regulates rsp5 ubiquitination activity in vivo and in vitro. PLoS One 8(9):e75372