RUB1/YDR139C Summary Help

Standard Name RUB1 1
Systematic Name YDR139C
Feature Type ORF, Verified
Description Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) (1, 2, 3, 4)
Name Description Related to UBiquitin 1
Chromosomal Location
ChrIV:733924 to 733618 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RUB1 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
70 total interaction(s) for 46 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 9
  • Biochemical Activity: 4
  • Protein-peptide: 4
  • Reconstituted Complex: 6
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 1
  • Negative Genetic: 18
  • Positive Genetic: 3
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 77
Molecular Weight (Da) 8,693
Isoelectric Point (pI) 6.26
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:733924 to 733618 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..149 733924..733776 2011-02-03 2000-12-01
Intron 150..222 733775..733703 2011-02-03 2000-12-01
CDS 223..307 733702..733618 2011-02-03 2000-12-01
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002546
References cited on this page View Complete Literature Guide for RUB1
1) Liakopoulos D, et al.  (1998) A novel protein modification pathway related to the ubiquitin system. EMBO J 17(8):2208-14
2) Lammer D, et al.  (1998) Modification of yeast Cdc53p by the ubiquitin-related protein rub1p affects function of the SCFCdc4 complex. Genes Dev 12(7):914-26
3) Hochstrasser M  (2000) Biochemistry. All in the ubiquitin family. Science 289(5479):563-4
4) Laplaza JM, et al.  (2004) Saccharomyces cerevisiae ubiquitin-like protein Rub1 conjugates to cullin proteins Rtt101 and Cul3 in vivo. Biochem J 377(Pt 2):459-67