RTT109/YLL002W Summary Help

Standard Name RTT109 1
Systematic Name YLL002W
Alias KIM2 , REM50
Feature Type ORF, Verified
Description Histone acetyltransferase; critical for cell survival in the presence of DNA damage during S phase; prevents hyper-amplification of rDNA; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p (1, 2, 3, 4, 5, 6 and see Summary Paragraph)
Name Description Regulator of Ty1 Transposition 1
Gene Product Alias KAT11 7
Chromosomal Location
ChrXII:146291 to 147601 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RTT109 GO evidence and references
  View Computational GO annotations for RTT109
Molecular Function
Manually curated
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
562 total interaction(s) for 355 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 10
  • Biochemical Activity: 17
  • Co-crystal Structure: 3
  • Co-purification: 2
  • Far Western: 1
  • FRET: 1
  • Reconstituted Complex: 13

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Negative Genetic: 200
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 15
  • Positive Genetic: 32
  • Synthetic Growth Defect: 219
  • Synthetic Lethality: 27
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 436
Molecular Weight (Da) 50,095
Isoelectric Point (pI) 10.18
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:146291 to 147601 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1311 146291..147601 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003925
SUMMARY PARAGRAPH for RTT109

Rtt109p is a histone acetyltransferase that associates with transcriptionally active genes and is required for proper acetylation of histone H3 at lysine 56 (H3K56), which occurs during both the premeiotic and mitotic S phase, and persists throughout DNA damage repair (8). Rtt109p directly catalyzes the H3K56 acetylation in a manner that is stimulated by histone chaperone Asf1p, which governs the substrate specificity of Rtt109p. Rtt109p also autoacetylates and can weakly acetylate Asf1p, but not histone H4 (3). Acetylation of H3K56 has been implicated in regulation of replication since H3K56 is transiently acetylated during S phase (3). This H3K56 acetylation plays a critical role in conferring resistance to replication stress, and is critical for cell survival in the presence of DNA damage during S phase (3).

Specific mutations in Rtt109p (D89A, D89N, and DD287-288AA) result in loss of H3K56 acetylation. D89 is absolutely essential for H3K56 acetylation, whereas DD287-288 are not essential but contribute (3). The rtt109 aspartate mutants listed above, as well as rtt109 null mutants, which lack H3K56 acetylation, exhibit slow growth, and are hypersensitive to agents that generate replication stress (methyl methanesulfonate, hydroxyurea, campothecin) and to phleomycin, but not to acute ionizing radiation treatment, similar to that of asf1 null and H3K56Q mutants (8, 3). Further, RTT109 deletion leads to an increased rate of gross chromosomal rearrangements as well as a hyperrecombination phenotype similar to that exhibited by an sgs1 null mutant (2). rtt109 null mutants exhibit synthetic genetic interactions with mutations in POL30 (PCNA), POL1 (DNA polymerase alpha), ORC2, and CDC45, all of which are involved in DNA replication (3).

RTT109 was originally identified in a genetic screen for regulators of transposition of the retrotransposon Ty1 (1). Homologs of RTT109 are present in Schizosaccharomyces pombe, Eremothecium gossypii, Candida glabrata, C. albicans, and Kluyveromyces lactis.

Last updated: 2007-03-13 Contact SGD

References cited on this page View Complete Literature Guide for RTT109
1) Scholes DT, et al.  (2001) Multiple regulators of Ty1 transposition in Saccharomyces cerevisiae have conserved roles in genome maintenance. Genetics 159(4):1449-65
2) Driscoll R, et al.  (2007) Yeast Rtt109 promotes genome stability by acetylating histone H3 on lysine 56. Science 315(5812):649-52
3) Han J, et al.  (2007) Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication. Science 315(5812):653-5
4) Jessulat M, et al.  (2008) Interacting proteins Rtt109 and Vps75 affect the efficiency of non-homologous end-joining in Saccharomyces cerevisiae. Arch Biochem Biophys 469(2):157-64
5) Fillingham J, et al.  (2008) Chaperone control of the activity and specificity of the histone H3 acetyltransferase Rtt109. Mol Cell Biol 28(13):4342-53
6) Ide S, et al.  (2013) Rtt109 prevents hyper-amplification of ribosomal RNA genes through histone modification in budding yeast. PLoS Genet 9(4):e1003410
7) Allis CD, et al.  (2007) New nomenclature for chromatin-modifying enzymes. Cell 131(4):633-6
8) Schneider J, et al.  (2006) Rtt109 is required for proper H3K56 acetylation: a chromatin mark associated with the elongating RNA polymerase II. J Biol Chem 281(49):37270-4