RTT107/YHR154W Summary Help

Standard Name RTT107 1
Systematic Name YHR154W
Alias ESC4 2
Feature Type ORF, Verified
Description Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress (1, 3, 4, 5, 6, 7)
Name Description Regulator of Ty1 Transposition 1
Chromosomal Location
ChrVIII:402966 to 406178 | ORF Map | GBrowse
Gene Ontology Annotations All RTT107 GO evidence and references
  View Computational GO annotations for RTT107
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 7 genes
Classical genetics
Large-scale survey
508 total interaction(s) for 352 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 28
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 18
  • Co-crystal Structure: 1
  • PCA: 3
  • Protein-peptide: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 21

Genetic Interactions
  • Negative Genetic: 188
  • Phenotypic Enhancement: 10
  • Phenotypic Suppression: 9
  • Positive Genetic: 113
  • Synthetic Growth Defect: 91
  • Synthetic Lethality: 15
  • Synthetic Rescue: 6

Expression Summary
Length (a.a.) 1,070
Molecular Weight (Da) 123,016
Isoelectric Point (pI) 7.73
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVIII:402966 to 406178 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3213 402966..406178 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001197
References cited on this page View Complete Literature Guide for RTT107
1) Scholes DT, et al.  (2001) Multiple regulators of Ty1 transposition in Saccharomyces cerevisiae have conserved roles in genome maintenance. Genetics 159(4):1449-65
2) Andrulis ED, et al.  (2004) One-hybrid screens at the Saccharomyces cerevisiae HMR locus identify novel transcriptional silencing factors. Genetics 166(1):631-5
3) Rouse J  (2004) Esc4p, a new target of Mec1p (ATR), promotes resumption of DNA synthesis after DNA damage. EMBO J 23(5):1188-97
4) Baldwin EL, et al.  (2005) Mms22p protects Saccharomyces cerevisiae from DNA damage induced by topoisomerase II. Nucleic Acids Res 33(3):1021-30
5) Roberts TM, et al.  (2006) Slx4 regulates DNA damage checkpoint-dependent phosphorylation of the BRCT domain protein Rtt107/Esc4. Mol Biol Cell 17(1):539-48
6) Leung GP, et al.  (2011) Rtt107 is required for recruitment of the SMC5/6 complex to DNA double strand breaks. J Biol Chem 286(29):26250-7
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76